Separation of endo- and exo-type cellulases using a new affinity chromatography method

A new efficient biospecific adsorbent for the purification of cellulases was prepared from p-aminobenzyl 1-thio-β-D-cellobioside and Affigel-10 (Biorad). The two exocellobiohydrolases, CBH I and CBH II, from Trichoderma reesei QM 9414 were adsorbed, whereas the endoglucanase activities were unretard...

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Veröffentlicht in:	FEBS letters 1984-04, Vol.169 (2), p.215-218
Hauptverfasser:	van Tilbeurgh, Herman, Bhikhabhai, Ramagauri, Göran Pettersson, L., Claeyssens, Marc
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity chromatography Analytical, structural and metabolic biochemistry Biological and medical sciences CBH, 1,4-β-glucan cellobiohydrolase (EC 3.2.1.91) Cellulase Endocellulase endocellulase, 1,4-β-glucan glucanohydrolase (EC 3.2.1.4) Enzyme purification Enzymes and enzyme inhibitors Exo-cellobiohydrolase Fundamental and applied biological sciences. Psychology HPLC, high-performance liquid chromatography Hydrolases Trichoderma reesei
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Beschreibung
Zusammenfassung:	A new efficient biospecific adsorbent for the purification of cellulases was prepared from p-aminobenzyl 1-thio-β-D-cellobioside and Affigel-10 (Biorad). The two exocellobiohydrolases, CBH I and CBH II, from Trichoderma reesei QM 9414 were adsorbed, whereas the endoglucanase activities were unretarded. The adsorbed CBH I can be displaced selectively by elution with lactose, whereas cellobiose elutes the CBH II.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(84)80321-X