Elution of Trichoderma reesei cellulase from cellulose by pH adjustment with sodium hydroxide

Up to 45% of bound cellulase (Avicelase) could be recovered from crystalline cellulose by pH adjustment with NaOH (0.008-0.01 M) to pH 10.0 (40 degree C). Enzyme was simultaneously desorbed and inactivated as the pH increased and both events occurred within 1 minute. Enzyme desorption and stability...

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Veröffentlicht in:	Biotechnology letters 1984-01, Vol.6 (6), p.369-374
Hauptverfasser:	OTTER, D. E, MUNRO, P. A, SCOTT, G. K, GEDDES, R
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology cellulase cellulose Enzyme engineering Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Trichoderma reesei Various methods and equipments
Online-Zugang:	Volltext
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Zusammenfassung:	Up to 45% of bound cellulase (Avicelase) could be recovered from crystalline cellulose by pH adjustment with NaOH (0.008-0.01 M) to pH 10.0 (40 degree C). Enzyme was simultaneously desorbed and inactivated as the pH increased and both events occurred within 1 minute. Enzyme desorption and stability were also temperature dependent and desorption increased to approximately 65% by adding detergents.
ISSN:	0141-5492 1573-6776
DOI:	10.1007/BF00138007