A simple and rapid technique for fluorescence staining of fungal nuclei

A simple and rapid technique for fluorescence staining of fungal nuclei

A technique for staining fungal nuclei using fluorescence stain Hoechst Dye 33258 in McIlvaine standard buffer of pH 7.26-7.44 is reported. It is a broad-spectrum fungal nuclear staining tool found to be effective on Agaricus bisporus, Alternaria helianthi, Fusarium oxysporum f. sp. lini, Penicilliu...

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Veröffentlicht in:Current microbiology 1984-01, Vol.10 (2), p.99-104
Hauptverfasser: Kangatharalingam, N, Ferguson, M W
Format: Artikel
Sprache:eng
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Zusammenfassung:A technique for staining fungal nuclei using fluorescence stain Hoechst Dye 33258 in McIlvaine standard buffer of pH 7.26-7.44 is reported. It is a broad-spectrum fungal nuclear staining tool found to be effective on Agaricus bisporus, Alternaria helianthi, Fusarium oxysporum f. sp. lini, Penicillium binellum, Pythium ultimum, Rhizoctonia solani , and Saccharomyces cerevisiae . Conidial nuclei of Alternaria helianthi, Fusarium oxysporum f. sp. lini , and Penicillium binellum also stained well.
ISSN:0343-8651
DOI:10.1007/BF01575767