Preparation of chlorophylls and pheophytins by isocratic liquid chromatography

Isocratic high-performance liquid chromatography (HPLC) with silica gel as a stationary phase provides a powerful means for rapid preparative isolation (on a 20-50 mg level) of chlorophylls and pheophytins. The purity and identity of the isolated pigments have been confirmed by complete elemental an...

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Veröffentlicht in:	Analytical chemistry (Washington) 1984-02, Vol.56 (2), p.251-256
Hauptverfasser:	Watanabe, Tadashi, Hongu, Akinori, Honda, Kenichi, Nakazato, Masataka, Konno, Mitsuo, Saitoh, Sadao
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences C.D chlorophyll Fundamental and applied biological sciences. Psychology Heterocyclic compounds, pigments high-performance liquid chromatography Other biological molecules pneophytin Tetrapyrrolic pigments U.V. spectroscopy
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Beschreibung
Zusammenfassung:	Isocratic high-performance liquid chromatography (HPLC) with silica gel as a stationary phase provides a powerful means for rapid preparative isolation (on a 20-50 mg level) of chlorophylls and pheophytins. The purity and identity of the isolated pigments have been confirmed by complete elemental analyses and analytical HPLC. UV-visible spectrometric data (in diethyl ether, acetone, and benzene) and CD spectra (in benzene) of the purified pigments are presented.
ISSN:	0003-2700 1520-6882
DOI:	10.1021/ac00266a030