Multiple forms of inorganic pyrophosphatase from the leaf of Amarantus blitum L

Previous studies on the distribution of inorganic pyrophosphatase in the leaves of plants have shown that the leaves of Amarantus blitum of the Amarantaceae family possess a very high level of this enzyme amongst fifty-one species of plants comprising twenty-two families tested. During purification of this enzyme from the leaf of A. blitum by ion exchange chromatography, the authors got two distinctly separated peaks with inorganic pyrophosphatase activity. In this paper the isolation and some properties of these two forms of enzyme from the leaf of A. blitum are reported.