Changes in DNA fragmentation during sperm preparation for intracytoplasmic sperm injection over time

Objective To compare the DNA fragmentation of semen samples established by terminal deoxynucleotide transferase–mediated dUTP nick-end labeling (TUNEL) after incubation in polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) for different time periods. Design Comparative prospective study. Setting Ce...

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Veröffentlicht in:	Fertility and sterility 2013-07, Vol.100 (1), p.69-74
Hauptverfasser:	Rougier, Natalia, M.D, Uriondo, Heydy, M.Sc, Papier, Sergio, M.D, Checa, Miguel Angel, M.D., Ph.D, Sueldo, Carlos, M.D, Alvarez Sedó, Cristian, M.Sc
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult centrifugation DNA Fragmentation Humans hyaluronic acid Infertility, Male - diagnosis Infertility, Male - therapy Internal Medicine intracytoplasmic sperm injection Male medicine Middle Aged Obstetrics and Gynecology patients Prospective Studies PVP semen Semen Analysis - methods Semen Analysis - trends Sperm DNA fragmentation Sperm Injections, Intracytoplasmic - methods Sperm Injections, Intracytoplasmic - trends spermatozoa Spermatozoa - physiology Time Factors
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Zusammenfassung:	Objective To compare the DNA fragmentation of semen samples established by terminal deoxynucleotide transferase–mediated dUTP nick-end labeling (TUNEL) after incubation in polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) for different time periods. Design Comparative prospective study. Setting Center for reproductive medicine. Patient(s) Twenty-seven semen samples from infertile patients. Intervention(s) None. Methods Semen analysis and DNA fragmentation assays (TUNEL) were performed. Two groups were established: A) normal TUNEL (
ISSN:	0015-0282 1556-5653
DOI:	10.1016/j.fertnstert.2013.03.005