Acetylation sites in histone H3 from Physarum polycephalum

Histone H3 from Physarum polycephalum was labelled with [ 3H]acetate in G2 phase of the cell cycle. Only histones H3 and H4 were labelled and the H4 was removed by chromatography. Sequential Edman degradation of labelled H3 showed that acetate was incorporated into residues 9, 14, 18 and 23 which co...

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Veröffentlicht in:	FEBS letters 1983-10, Vol.162 (2), p.416-419
Hauptverfasser:	Waterborg, Jaap H., Matthews, Harry R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetyl-lysine acetylation Acetylation site Analytical, structural and metabolic biochemistry Biological and medical sciences Fundamental and applied biological sciences. Psychology H3 sequence conservation Higher eukaryote Histone H3 Holoproteins Nuclear proteins Physarum polycephalum Proteins
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Zusammenfassung:	Histone H3 from Physarum polycephalum was labelled with [ 3H]acetate in G2 phase of the cell cycle. Only histones H3 and H4 were labelled and the H4 was removed by chromatography. Sequential Edman degradation of labelled H3 showed that acetate was incorporated into residues 9, 14, 18 and 23 which correspond to the sites of acetyl-lysine determined in histones H3 from other organisms. The results confirm the sequence conservation of H3 and support the notion that data on H3 acetylation, obtained with Physarum, can be extrapolated to higher eukaryotes.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(83)80798-4