Biological activity and physicochemical properties of thermally activated water types: Mollusk-based bioassay

Physicochemical and bioassay methods are used to carry out a comprehensive study of the properties of two types of activated waters: “thermally activated,” i.e., thermal water, referred to as “white spring” or “cold boiling water” in folk medicine, and “melt” water after quick freeze. The test object for bioassay were apple shell mollusks Pomacea canaliculata from a laboratory culture used to carry out patented and widely tested methods of freshwater quality bioassay. Oxygen and hydrogen peroxide concentrations and pH were determined in water. The integral biological activity of water was evaluated by a method based on the use of absorption spectra to evaluate the rate of redox transformation in the quinone-hydroquinol system. Similar studies were carried out with three types of reference samples: settled original tap water, the same water cooled to the freezing point, and water artificially enriched with hydrogen peroxide. The water activated by thermal impact has been found to have higher biological activity than the original tap water.