Rapid transformation of Chlamydomonas reinhardtii without cell-wall removal

Chlamydomonas reinhardtii is widely used to study many biological processes including biofuel production. Here, we present a rapid transformation technique for cell-walled Chlamydomonas strains without cell-wall removal using a square electric pulses-generating electroporator. This method could be a...

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Veröffentlicht in:	Journal of bioscience and bioengineering 2013-06, Vol.115 (6), p.691-694
Hauptverfasser:	Yamano, Takashi, Iguchi, Hiro, Fukuzawa, Hideya
Format:	Artikel
Sprache:	eng
Schlagworte:	Algae biofuels Biological and medical sciences Biotechnology Cell Wall cell walls Chlamydomonas reinhardtii Chlamydomonas reinhardtii - genetics Electroporation Electroporation - methods Fundamental and applied biological sciences. Psychology Square electric pulse strains Transformation Transformation, Genetic
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Beschreibung
Zusammenfassung:	Chlamydomonas reinhardtii is widely used to study many biological processes including biofuel production. Here, we present a rapid transformation technique for cell-walled Chlamydomonas strains without cell-wall removal using a square electric pulses-generating electroporator. This method could be applied to transformation of other industrially useful algae by optimizing the electric conditions.
ISSN:	1389-1723 1347-4421
DOI:	10.1016/j.jbiosc.2012.12.020