Immobilization of alpha-amylase, glucoamylase and glucose isomerase on CNBr activated Sepharose-6MB

Two enzymes (glycoamylase from Rhizopus sp. and glucose isomerase from Streptomyces fradiae ) or three enzymes (the above two and alpha -amylase from Bacillus subtilis ) were coupled to CNBr activated Sepharose-6MB. Some of the parameters such as the pH and temperature activity relationships of the immobilized enzyme systems are described. A 5% soluble starch solution was converted by the enzyme system into a mixture of glucose and fructose within 6 hr at 60 degree C.