Proteome variability among Helicobacter pylori isolates clustered according to genomic methylation
Aims To understand whether the variability found in the proteome of Helicobacter pylori relates to the genomic methylation, virulence and associated gastric disease. Methods and Results We applied the Minimum‐Common‐Restriction‐Modification (MCRM) algorithm to genomic methylation data of 30 Portugue...
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Veröffentlicht in: | Journal of applied microbiology 2013-06, Vol.114 (6), p.1817-1832 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Aims
To understand whether the variability found in the proteome of Helicobacter pylori relates to the genomic methylation, virulence and associated gastric disease.
Methods and Results
We applied the Minimum‐Common‐Restriction‐Modification (MCRM) algorithm to genomic methylation data of 30 Portuguese H. pylori strains, obtained by genome sensitivity to Type II restriction enzymes' digestion. All the generated dendrograms presented three clusters with no association with gastric disease. Comparative analysis of two‐dimensional gel electrophoresis (2DE) maps obtained for total protein extracts of 10 of these strains, representative of the three main clusters, revealed that among 70 matched protein spots (in a universe of 300), 16 were differently abundant (P |
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ISSN: | 1364-5072 1365-2672 |
DOI: | 10.1111/jam.12187 |