Effect of dietary supplementation of prebiotics and probiotics on intestinal microarchitecture in broilers reared under cyclic heat stress
Summary This study was designed to evaluate the effect of dietary supplementation of prebiotics, mannanoligosaccharides (MOS) and/or probiotics (LBP) on intraepithelial lymphocytes (IEL) count, goblet cells (GC) count and differentiation and intestinal micro‐architecture in broilers reared under cyc...
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Veröffentlicht in: | Journal of animal physiology and animal nutrition 2013-05, Vol.97 (s1), p.68-73 |
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Format: | Artikel |
Sprache: | eng |
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This study was designed to evaluate the effect of dietary supplementation of prebiotics, mannanoligosaccharides (MOS) and/or probiotics (LBP) on intraepithelial lymphocytes (IEL) count, goblet cells (GC) count and differentiation and intestinal micro‐architecture in broilers reared under cyclic heat stress. Day‐old broilers (n = 250) were randomly divided into five groups. Fifty birds were reared within the thermoneutral zone (TNZ). Remaining birds were subjected to cyclic heat stress from day 21 to 42 (35° C, 75% RH, 8 h/d). The birds were fed corn‐soy‐based basal diet or the same diet supplemented with 0.5% MOS (HS‐MOS), or 0.1% LBP (HS‐LBP), or their combination (HS‐SYN). The birds were slaughtered on day 42. Tissue samples were collected from mid‐duodenum, jejunum and ileum, and stained with haematoxylin and eosin or combined Alcian blue and PAS technique. All differences were considered significant at p |
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ISSN: | 0931-2439 1439-0396 |
DOI: | 10.1111/jpn.12041 |