Single-drop microextraction as a powerful pretreatment tool for capillary electrophoresis: A review

. [Display omitted] ► SDME is a convenient and powerful preconcentration and sample cleanup method for CE. ► SDME-CE has been applied to the analysis of target analytes in complex matrices. ► SDME-CE has been hyphenated with other on-line preconcentration techniques in CE. Single drop microextraction (SDME) is a convenient and powerful preconcentration and sample cleanup method for capillary electrophoresis (CE). In SDME, analytes are typically extracted from a sample donor solution into an acceptor drop hanging at the inlet tip of a capillary. The enriched drop is then introduced to the capillary for CE analysis. Since the volume of the acceptor drop can be as small as a few nanoliters, the consumption of solvents can be minimized and the preconcentration effect is enhanced. In addition, by covering the acceptor phase with an organic layer or by using an organic acceptor phase, inorganic ions such as salts in the sample solution can be blocked from entering the acceptor phase, providing desalting effects. Here, we describe the basic principles and instrumentation for SDME and its coupling with CE. We also review recent developments and applications of SDME-CE.