Inhibition of human B-cell development into plasmablasts by histone deacetylase inhibitor valproic acid

In particular, VPA ameliorates the pathogenesis of experimental autoimmune encephalomyelitis,E1 decreases the incidence and severity of collagen-induced arthritis,E2 and attenuates inflammation in autoimmune neuritis.E3In vitro treatment of human dendritic cells with VPA impairs their migratory capability and potential to effectively activate T cells.3 Furthermore, HDAC inhibition suppresses T-cell proliferation,E4 increases TH2 cytokine production,E5 and induces suppressive function of regulatory T cells.E6 Humoral immune responses against bacteria are maintained by immunoglobulin-secreting plasma cells and memory B cells. [...]membranes were incubated in SuperSignal West Femto Maximum Sensitivity Substrate or in SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, Waltham, Mass).