Separation of the subunits of crotoxin by high-performance liquid chromatography

Crotoxin is the major toxin in the venom of the Brazilian rattlesnake (Crotalus durissus terrificus ). It is a complex of two subunits associated non-covalently: a basic protein (crotoxin PLA or crotoxin B) which carries the phospholipase (PLA) activity and an acidic protein (crotapotin or crotoxin...

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Veröffentlicht in:	Journal of Chromatography A 1983-01, Vol.259 (2), p.375-377
Hauptverfasser:	Wu, S H, Chang, F H, Tzeng, M C
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Chromatography, High Pressure Liquid Crotalid Venoms - analysis Crotalid Venoms - isolation & purification Crotalus durissus terrificus crotoxin Crotoxin - isolation & purification Fundamental and applied biological sciences. Psychology Miscellaneous Proteins venom
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Beschreibung
Zusammenfassung:	Crotoxin is the major toxin in the venom of the Brazilian rattlesnake (Crotalus durissus terrificus ). It is a complex of two subunits associated non-covalently: a basic protein (crotoxin PLA or crotoxin B) which carries the phospholipase (PLA) activity and an acidic protein (crotapotin or crotoxin A) which has no enzymic activity and is not toxic. In this work the separation of the two subunits of crotoxin by high-performance liquid chromatography (HPLC) was investigated.
ISSN:	0021-9673
DOI:	10.1016/S0021-9673(01)88024-9