A simple method for the removal of mycoplasma contamination from paramyxovirus stocks

A simple and quick method of detecting mycoplasmas in virus stocks using the fluorochrome Hoechst 33258 is described. Different methods of removal of mycoplasmas from stocks are discussed. The simple but effective method using gentamicin (0.2 mg/ml) or chloramphenicol (5 μg/ml) is demonstrated and c...

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Veröffentlicht in:	Journal of virological methods 1983-01, Vol.6 (3), p.127-134
Hauptverfasser:	Simpson, E.J., Cosby, S.L., Rima, B.K., Martin, S.J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Anti-Bacterial Agents - pharmacology Bromouracil - pharmacology Cells, Cultured Chloramphenicol - pharmacology Gentamicins - pharmacology Kanamycin - pharmacology Leucomycins - pharmacology Mycoplasma Mycoplasma - drug effects Mycoplasma - genetics mycoplasma detection and removal Paramyxoviridae - growth & development Paramyxoviridae - isolation & purification RNA, Bacterial - analysis Tylosin virus stocks
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