A simple method for the removal of mycoplasma contamination from paramyxovirus stocks

A simple method for the removal of mycoplasma contamination from paramyxovirus stocks

A simple and quick method of detecting mycoplasmas in virus stocks using the fluorochrome Hoechst 33258 is described. Different methods of removal of mycoplasmas from stocks are discussed. The simple but effective method using gentamicin (0.2 mg/ml) or chloramphenicol (5 μg/ml) is demonstrated and c...

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Veröffentlicht in:Journal of virological methods 1983-01, Vol.6 (3), p.127-134
Hauptverfasser: Simpson, E.J., Cosby, S.L., Rima, B.K., Martin, S.J.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Anti-Bacterial Agents - pharmacology
Bromouracil - pharmacology
Cells, Cultured
Chloramphenicol - pharmacology
Gentamicins - pharmacology
Kanamycin - pharmacology
Leucomycins - pharmacology
Mycoplasma
Mycoplasma - drug effects
Mycoplasma - genetics
mycoplasma detection and removal
Paramyxoviridae - growth & development
Paramyxoviridae - isolation & purification
RNA, Bacterial - analysis
Tylosin
virus stocks
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Zusammenfassung:A simple and quick method of detecting mycoplasmas in virus stocks using the fluorochrome Hoechst 33258 is described. Different methods of removal of mycoplasmas from stocks are discussed. The simple but effective method using gentamicin (0.2 mg/ml) or chloramphenicol (5 μg/ml) is demonstrated and chosen as the most efficient as judged using both the Hoechst stain and the direct assessment of mycoplasma RNA species labelled with [5- 3H]uridine.
ISSN:0166-0934
1879-0984
DOI:10.1016/0166-0934(83)90024-1