Effects of dinitrophenol, actinomycin-D and puromycin on encystment of the ameboflagellate, Naegleria gruberi
Populations of encysting amebas, N. gruberi , were exposed to either 1 x 10 super(-4)M dinitrophenol, or to 1 x 10 super(-5)M actinomycin-d, or to 5 x 10 super(-4)M puromycin in conditions that would otherwise (in controls) produce maximal encystment is 48 h. Dinitrophenol retarded the rate of encys...
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Veröffentlicht in: | Acta protozoologica 1982-01, Vol.21 (3-4), p.213-220 |
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Sprache: | eng |
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Zusammenfassung: | Populations of encysting amebas, N. gruberi , were exposed to either 1 x 10 super(-4)M dinitrophenol, or to 1 x 10 super(-5)M actinomycin-d, or to 5 x 10 super(-4)M puromycin in conditions that would otherwise (in controls) produce maximal encystment is 48 h. Dinitrophenol retarded the rate of encystment, but did not halt it, so that 67.3% of the population encysted in 48 h while in accompanying control populations over 80% had encysted. Puromycin completely blocked the encystment and no amebas progressed beyond the rounded, initial, precystic state and no cyst walls formed. Actinomycin-d accelerated the rate of encystment. It is suggested that dinitrophenol reduces the rate of encystment by reducing the amount of available adenosine triphosphate (ATP) and thereby reduces the amount of available energy to support the enzyme-systems involved in the encystment processes. Puromycin is considered to inhibit transcription of the messenger-RNA codes. Actinomycin-d is considered to stimulate the release of stored messenger-RNAs from the nucleolus, thereby accelerating the transcription of and polymerization of the enzyme-systems required to promote the formation of the cyst. |
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ISSN: | 0065-1583 |