Isolation of a human anti-haemophilic factor IX cDNA clone using a unique 52-base synthetic oligonucleotide probe deducted from the amino acid sequence of bovine factor IX

Isolation of a human anti-haemophilic factor IX cDNA clone using a unique 52-base synthetic oligonucleotide probe deducted from the amino acid sequence of bovine factor IX

A unique 52mer oligonucleotide deduced from the amino acid sequence of bovine Factor IX was synthesized and used as a probe to screen a human liver cDNA bank. The Factor IX clone isolated shows 5 differences in nucleotide and deduced amino acid sequence as compared to a previously isolated clone. In...

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Veröffentlicht in:Nucleic acids research 1983-01, Vol.11 (8), p.2325-2335
Hauptverfasser: Jaye, M, de la Salle, H, Schamber, F, Balland, A, Kohli, V, Findeli, A, Tolstoshev, P, Lecocq, J-P
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Sprache:eng
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Zusammenfassung:A unique 52mer oligonucleotide deduced from the amino acid sequence of bovine Factor IX was synthesized and used as a probe to screen a human liver cDNA bank. The Factor IX clone isolated shows 5 differences in nucleotide and deduced amino acid sequence as compared to a previously isolated clone. In addition, precisely one codon has been deleted.
ISSN:0305-1048