An environmentally sensitive fluorescent purine nucleoside that changes emission wavelength upon hybridization

An environmentally sensitive fluorescent purine nucleoside that changes emission wavelength upon hybridization

C7-naphthylethynylated 8-aza-7-deaza-2'-deoxyguanosine (na)G was synthesized and its photophysical properties were examined. The fluorescent nucleoside exhibited solvatofluorochromic properties (Δλ(fl)(max) = 67 nm). An ODN probe containing (na)G forms a stable base pair only with C and discrim...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2013-06, Vol.49 (50), p.5684-5686
Hauptverfasser: Saito, Yoshio, Suzuki, Azusa, Okada, Yuji, Yamasaka, Yuki, Nemoto, Nobukatsu, Saito, Isao
Format: Artikel
Sprache:eng
Schlagworte:
Deoxyguanosine - analogs & derivatives
Deoxyguanosine - chemistry
DNA Mutational Analysis
Fluorescent Dyes - chemistry
Mutation
Nucleic Acid Hybridization
Oligonucleotide Probes - chemistry
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Zusammenfassung:C7-naphthylethynylated 8-aza-7-deaza-2'-deoxyguanosine (na)G was synthesized and its photophysical properties were examined. The fluorescent nucleoside exhibited solvatofluorochromic properties (Δλ(fl)(max) = 67 nm). An ODN probe containing (na)G forms a stable base pair only with C and discriminates structural changes such as mismatches and deletions by a distinct change in its emission wavelength.
ISSN:1359-7345
1364-548X
DOI:10.1039/c3cc42605j