A protein kinase assay based on FRET between quantum dots and fluorescently-labeled peptides

A novel protein kinase assay was developed, based on FRET between QDs and fluorescently-labeled substrate peptides. The negatively charged QDs recognize the change in net charge of the peptide upon phosphorylation. Despite its simple mechanism, this assay is sensitive and robust enough to be applied...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2013-06, Vol.49 (49), p.5592-5594
Hauptverfasser:	Shiosaki, Shujiro, Nobori, Takanobu, Mori, Takeshi, Toita, Riki, Nakamura, Yuta, Kim, Chan Woo, Yamamoto, Tatsuhiro, Niidome, Takuro, Katayama, Yoshiki
Format:	Artikel
Sprache:	eng
Schlagworte:	Dose-Response Relationship, Drug Fluorescence Fluorescence Resonance Energy Transfer Peptides - chemistry Protein Kinase Inhibitors - pharmacology Protein Kinases - analysis Protein Kinases - metabolism Quantum Dots Structure-Activity Relationship
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	A novel protein kinase assay was developed, based on FRET between QDs and fluorescently-labeled substrate peptides. The negatively charged QDs recognize the change in net charge of the peptide upon phosphorylation. Despite its simple mechanism, this assay is sensitive and robust enough to be applied to the evaluation of protein kinase inhibitors.
ISSN:	1359-7345 1364-548X
DOI:	10.1039/c3cc41680a