Surface Enhanced Resonance Raman Scattering (SERRS)-A First Example of its Use in Multiplex Genotyping

Accurate multiplex genotyping of the mutational status of the cystic fibrosis gene with the sensitive and selective SERRS technique is reported. This new assay does not require separation of the various alleles and shows that SERRS is a realistic alternative detection technique for use in modern DNA...

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Veröffentlicht in:	Chemphyschem 2001-12, Vol.2 (12), p.746-748
Hauptverfasser:	Graham, Duncan, Mallinder, Benjamin J., Whitcombe, David, Smith, W. Ewen
Format:	Artikel
Sprache:	eng
Schlagworte:	Alleles Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry Biological and medical sciences colloids Cystic Fibrosis - genetics Cystic Fibrosis - metabolism Cystic Fibrosis - pathology DNA analysis DNA Primers - chemistry DNA Primers - metabolism Fundamental and applied biological sciences. Psychology Genotype Homozygote Humans Multiplex Polymerase Chain Reaction nucleic acids Oligonucleotide Array Sequence Analysis Raman spectroscopy Rhodamines - chemistry sequence determination Spectrum Analysis, Raman
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Beschreibung
Zusammenfassung:	Accurate multiplex genotyping of the mutational status of the cystic fibrosis gene with the sensitive and selective SERRS technique is reported. This new assay does not require separation of the various alleles and shows that SERRS is a realistic alternative detection technique for use in modern DNA assays.
ISSN:	1439-4235 1439-7641
DOI:	10.1002/1439-7641(20011217)2:12<746::AID-CPHC746>3.0.CO;2-Q