Sonic hedgehog inhibition induces mouse embryonic stem cells to differentiate toward definitive endoderm

In the experimental group (shh inhibited group), there were significant decreases in the expression of Oct4, Nanog, Shh, GATA4, Brachyury and Goosecoid, while increases were observed for TAT and Pdx1. The expression of Sox17 did not differ between two control and experimental groups. In experimental...

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Veröffentlicht in:	Indian journal of experimental biology 2013-03, Vol.51 (3), p.201-207
Hauptverfasser:	Ghanbari, Ali, Khazaei, Mozafar, Hashemi-Tabar, Mahmoud, Rabzia, Arezou, Fathi, Fardin, Bayat, Parvin-Dokht
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Differentiation Cell Line Cell Lineage Dithizone - pharmacology DNA Primers Embryonic Stem Cells - cytology Endoderm - metabolism Gene Expression Regulation, Developmental Hedgehog Proteins - metabolism Homeodomain Proteins - metabolism Immunohistochemistry Mesoderm - metabolism Mice Microscopy, Fluorescence Octamer Transcription Factor-3 - metabolism Reverse Transcriptase Polymerase Chain Reaction
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Zusammenfassung:	In the experimental group (shh inhibited group), there were significant decreases in the expression of Oct4, Nanog, Shh, GATA4, Brachyury and Goosecoid, while increases were observed for TAT and Pdx1. The expression of Sox17 did not differ between two control and experimental groups. In experimental group, the amount of GSC positive cells was somehow lower but it seems that there was no difference for Sox17. Shh inhibition induces ESCs to differentiate toward definitive endoderm by committing mesendodermal lineages.
ISSN:	0019-5189