Development and pre-clinical assessment of a 73 kD chimeric fusion protein as a defined sub-unit vaccine for leprosy

Highlights ► We produce a chimeric fusion protein incorporating selected Mycobacterium leprae antigens. ► We demonstrate protein recognition by IgG in MB leprosy patient sera. ► We demonstrate that fusion protein stimulates IFNγ production. ► We elicit a pluripotent Ag-specific Th1 response by inclu...

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Veröffentlicht in:	Vaccine 2013-01, Vol.31 (5), p.813-819
Hauptverfasser:	Duthie, Malcolm S, Sampaio, Lucas H, Oliveira, Regiane M, Raman, Vanitha S, O’Donnell, Joanne, Bailor, H. Remy, Ireton, Greg C, Sousa, Ana Lucia M, Stefani, Mariane M.A, Reed, Steven G
Format:	Artikel
Sprache:	eng
Schlagworte:	Adjuvants, Immunologic - administration & dosage Adolescent Adult Aged Aged, 80 and over Allergy and Immunology Animals Antibodies, Bacterial - blood Antigens, Bacterial - genetics Antigens, Bacterial - immunology Applied microbiology Bacterial diseases Bacterial Vaccines - administration & dosage Bacterial Vaccines - genetics Bacterial Vaccines - immunology Bacteriology Biological and medical sciences Female Fundamental and applied biological sciences. Psychology Human bacterial diseases Humans Immunoglobulin G - blood Infectious diseases Interferon-gamma - secretion Leprosy Leprosy - immunology Leprosy - prevention & control Leukocytes, Mononuclear - immunology Male Medical sciences Mice Mice, Inbred C57BL Microbiology Middle Aged Miscellaneous Mycobacterium leprae Mycobacterium leprae - immunology Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - immunology Th1 Cells - immunology Tropical bacterial diseases Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccines, Synthetic - administration & dosage Vaccines, Synthetic - genetics Vaccines, Synthetic - immunology Young Adult
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Beschreibung
Zusammenfassung:	Highlights ► We produce a chimeric fusion protein incorporating selected Mycobacterium leprae antigens. ► We demonstrate protein recognition by IgG in MB leprosy patient sera. ► We demonstrate that fusion protein stimulates IFNγ production. ► We elicit a pluripotent Ag-specific Th1 response by including a TLR4L- adjuvant. ► We show immunization inhibits M. leprae -induced inflammation in mice.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2012.11.073