Selective Isotope Labelling of Leucine Residues by Using α-Ketoacid Precursor Compounds
You can have one without the other: A new metabolic precursor compound can selectively introduce 13C and 2H patterns at leucine residues in proteins in cell‐based expression systems without interfering with valine metabolism. The protocol provides selectively labelled macromolecules well suited for...
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| Veröffentlicht in: | Chembiochem : a European journal of chemical biology 2013-05, Vol.14 (7), p.818-821 |
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| container_issue | 7 |
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| container_title | Chembiochem : a European journal of chemical biology |
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| creator | Lichtenecker, Roman J. Coudevylle, Nicolas Konrat, Robert Schmid, Walther |
| description | You can have one without the other: A new metabolic precursor compound can selectively introduce 13C and 2H patterns at leucine residues in proteins in cell‐based expression systems without interfering with valine metabolism. The protocol provides selectively labelled macromolecules well suited for probing structure and dynamics in high‐molecular‐weight proteins by NMR spectroscopy. |
| doi_str_mv | 10.1002/cbic.201200737 |
| format | Article |
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| ispartof | Chembiochem : a European journal of chemical biology, 2013-05, Vol.14 (7), p.818-821 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Carbon Isotopes cell-based protein expression Isotope Labeling isotopic labeling Keto Acids - chemistry Leucine - chemistry Magnetic Resonance Spectroscopy metabolic precursors Molecular Structure NMR spectroscopy proteins Protons |
| title | Selective Isotope Labelling of Leucine Residues by Using α-Ketoacid Precursor Compounds |
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