Selective Isotope Labelling of Leucine Residues by Using α-Ketoacid Precursor Compounds

Selective Isotope Labelling of Leucine Residues by Using α-Ketoacid Precursor Compounds

You can have one without the other: A new metabolic precursor compound can selectively introduce 13C and 2H patterns at leucine residues in proteins in cell‐based expression systems without interfering with valine metabolism. The protocol provides selectively labelled macromolecules well suited for...

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Veröffentlicht in:Chembiochem : a European journal of chemical biology 2013-05, Vol.14 (7), p.818-821
Hauptverfasser: Lichtenecker, Roman J., Coudevylle, Nicolas, Konrat, Robert, Schmid, Walther
Format: Artikel
Sprache:eng
Schlagworte:
Carbon Isotopes
cell-based protein expression
Isotope Labeling
isotopic labeling
Keto Acids - chemistry
Leucine - chemistry
Magnetic Resonance Spectroscopy
metabolic precursors
Molecular Structure
NMR spectroscopy
proteins
Protons
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Beschreibung
Zusammenfassung:You can have one without the other: A new metabolic precursor compound can selectively introduce 13C and 2H patterns at leucine residues in proteins in cell‐based expression systems without interfering with valine metabolism. The protocol provides selectively labelled macromolecules well suited for probing structure and dynamics in high‐molecular‐weight proteins by NMR spectroscopy.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.201200737