Vectorial charge transfer reactions on the donor side of manganese-depleted and reconstituted photosystem 2 core complexes

The light-induced functioning of photosystem 2 (PS 2) is directly linked to the translocation of both electrons and protons across the membrane, which results in the formation of transmembrane electric potential difference (ΔΨ). Generation of ΔΨ due to S-state transitions of the water oxidation comp...

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Veröffentlicht in:Biochemistry (Moscow) 2013-04, Vol.78 (4), p.395-402
Hauptverfasser: Petrova, I. O., Kurashov, V. N., Zaspa, A. A., Semenov, A. Yu, Mamedov, M. D.
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Sprache:eng
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Zusammenfassung:The light-induced functioning of photosystem 2 (PS 2) is directly linked to the translocation of both electrons and protons across the membrane, which results in the formation of transmembrane electric potential difference (ΔΨ). Generation of ΔΨ due to S-state transitions of the water oxidation complex was demonstrated for the first time in Mn-depleted and reconstituted PS 2 core complexes incorporated into liposomes. The kinetics and relative amplitudes of the electrogenic reactions in dark-adapted samples during S 1 →S 2 , S 2 →S 3 , and S 4 →S 0 transitions in response to the first, second and third laser flashes were comparable to those obtained in the intact PS 2 core particles. These results expand current understanding of the nature and mechanisms of electrogenic (vectorial) reactions due to a charge transfer on the donor side of PS 2.
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297913040081