Immobilization of Lysozyme on Biomass Charcoal Powder Derived from Plant Biomass Wastes

Biomass charcoal powder (BCP) was used as a carrier matrix for immobilization of chicken egg white lysozyme. BCP was derived from plant biomass wastes such as dumped adzuki beans by pyrolysis without combustion under nitrogen atmosphere and grinding with a jet mill. The amount of lysozyme immobilized on BCP of adzuki beans by adsorption was 11 mu mol/g (0.16 g/g) at pH 7.0. The optimum pH values for free and immobilized lysozyme activities were 6.8 and 7.2, respectively. The optimum temperature for both free and immobilized lysozyme activities was 25 degree C. The half-life of immobilized lysozyme exhibited 1.8-fold compared to that of free lysozyme at 5 degree C. Moreover, the half life of immobilized lysozyme was 7 times greater than that of lysozyme at 90 degree C.