Physalin B inhibits Trypanosoma cruzi infection in the gut of Rhodnius prolixus by affecting the immune system and microbiota

[Display omitted] ► The natural compound, physalin B, inhibited parasite development in insect vector. ► Physalin B treated insects presented higher microbiota in the gut. ► Physalin B (topic and contact) diminished antibacterial activity in infected insects. ► Physalin B (oral) produced higher levels of nitrite and nitrate in infected insects. Physalin B is a natural secosteroidal, extracted from the Solanaceae plant, Physalis angulata, and it presents immune-modulator effects on the bloodsucking bug, Rhodnius prolixus. In this work, R. prolixus was treated with physalin B at a concentration of 1mg/ml of blood meal (oral application), or 20ng/insect (applied topically) or 57ng/cm2 of filter paper (contact treatment), and infected with Trypanosoma cruzi Dm28c clone (2×106 epimastigotes/insect). The three types of applications significantly decreased the number of T. cruzi Dm28c in the gut comparing with the non-treated infected insects (controls). All groups of infected insects treated with physalin B had higher numbers of bacterial microbiota in the gut than the non-treated controls infected with T. cruzi. We observed that the infected physalin B insects with topical and contact treatments had a lower antibacterial activity in the gut when compared with control infected insects. Furthermore, infected insects with the physalin B oral treatment produced higher levels of nitrite and nitrate in the gut than control infected insects. These results demonstrate that physalin B decreases the T. cruzi transmission by inhibiting the parasite development in the insect vector R. prolixus. Herein the importance of physalin B modulation on the immune system and microbiota population in terms of parasite development and transmission are discussed.