Peach (Prunus persica) extract inhibits angiotensin II-induced signal transduction in vascular smooth muscle cells

► Peach extract inhibits an intracellular Ca2+ elevation induced by angiotensin II. ► Peach extract inhibits reactive oxygen species production induced by angiotensin II. ► Angiotensin II-induced signal transduction events are inhibited by peach extract. Angiotensin II (Ang II) is a vasoactive hormone that has been implicated in cardiovascular diseases. Here, the effect of peach, Prunus persica L. Batsch, pulp extract on Ang II-induced intracellular Ca2+ mobilization, reactive oxygen species (ROS) production and signal transduction events in cultured vascular smooth muscle cells (VSMCs) was investigated. Pretreatment of peach ethyl acetate extract inhibited Ang II-induced intracellular Ca2+ elevation in VSMCs. Furthermore, Ang II-induced ROS generation, essential for signal transduction events, was diminished by the peach ethyl acetate extract. The peach ethyl acetate extract also attenuated the Ang II-induced phosphorylation of epidermal growth factor receptor and myosin phosphatase target subunit 1, both of which are associated with atherosclerosis and hypertension. These results suggest that peach ethyl acetate extract may have clinical potential for preventing cardiovascular diseases by interfering with Ang II-induced intracellular Ca2+ elevation, the generation of ROS, and then blocking signal transduction events.