Light-Controlled Modulation of Gene Expression Using Polyamidoamine Formulations
A promising method that offers both time- and site-specific delivery of macromolecules is photochemical internalization technology (PCI). Here, we have characterized various polyamidoamine (PAMAM) carriers [generation (G) 0–7], for light-directed delivery of nucleic acids in vitro by the use of PCI...
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Veröffentlicht in: | Nucleic acid therapeutics 2013-04, Vol.23 (2), p.16-165 |
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Sprache: | eng |
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Zusammenfassung: | A promising method that offers both time- and site-specific delivery of macromolecules is photochemical internalization technology (PCI). Here, we have characterized various polyamidoamine (PAMAM) carriers [generation (G) 0–7], for light-directed delivery of nucleic acids
in vitro
by the use of PCI technology. A number of parameters for optimal delivery of nucleic acids into human cancer cells, that is, various light-doses, carrier-doses, and small interfering RNA (siRNA)/messenger RNA (mRNA) doses were investigated for either up- or down-regulation of enhanced green fluorescent protein (EGFP) gene expression. In summary, our results showed in an osteosarcoma cell line (OHS) [EGFP] model system the possibility for efficient light-directed siRNA silencing (>80% silencing) when using PAMAM G3 to G7 as carriers. Surprisingly, no EGFP mRNA up-regulation was detected either with or without PCI after EGFP mRNA/PAMAM (G0–G7) transfection in standard OHS cells. We have here identified properties for PAMAM formulations enabling light-directed siRNA delivery with the aim of developing a site-specific strategy for delivery of nucleic acids
in vivo
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ISSN: | 2159-3337 2159-3345 |
DOI: | 10.1089/nat.2012.0413 |