Genetic Diversity of Natural and Planted Populations of Bretschneidera sinensis from Nanling Region

Genetic Diversity of Natural and Planted Populations of Bretschneidera sinensis from Nanling Region

为评价伯乐树迁地保护林的遗传完整性,采用ISSR分子标记技术对南岭山地伯乐树2个人工种群和4个天然种群的遗传多样性进行分析比较。结果显示：7条引物共检测出86个条带,其中62条为多态条带;各种群多态条带百分比（PPB）和Shannon表型多样性指数（HPOP）分别为36.05%～53.49%和0.2040～0.3079;天然种群PPB和HPOP分别为70.93%和0.3882,高于人工种群（PPB=53.49%,HPOP=0.2941）。分子方差分析（AMOVA）表明,天然种群的遗传分化（ΦST=0.3407）明显高于人工种群（ΦST=0.2248）。研究结果认为,伯乐树在物种水平和种群水平都...

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Veröffentlicht in:Linye kexue (1979) 2012-12, Vol.48 (12), p.45-52
Hauptverfasser: Liang Yan, Central South University of Forestry and Technology, Changsha (China),The Laboratory of Forestry Genetics, Xu Gangbiao, Central South University of Forestry and Technology, Changsha (China),The Laboratory of Forestry Genetics, Zhang Heping, Central South University of Forestry and Technology, Changsha (China),The Laboratory of Forestry Genetics
Format: Artikel
Sprache:chi
Schlagworte:
AMELIORATION DES PLANTES
FITOGENETICA
FITOMEJORAMIENTO
GENETIC RESOURCES
http://www.fao.org/aos/agrovoc#c_3218
http://www.fao.org/aos/agrovoc#c_49985
http://www.fao.org/aos/agrovoc#c_5956
PHYTOGENETIQUE
PLANT BREEDING
PLANT GENETICS
RECURSOS GENETICOS
RESSOURCE GENETIQUE
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Zusammenfassung:为评价伯乐树迁地保护林的遗传完整性,采用ISSR分子标记技术对南岭山地伯乐树2个人工种群和4个天然种群的遗传多样性进行分析比较。结果显示：7条引物共检测出86个条带,其中62条为多态条带;各种群多态条带百分比（PPB）和Shannon表型多样性指数（HPOP）分别为36.05%～53.49%和0.2040～0.3079;天然种群PPB和HPOP分别为70.93%和0.3882,高于人工种群（PPB=53.49%,HPOP=0.2941）。分子方差分析（AMOVA）表明,天然种群的遗传分化（ΦST=0.3407）明显高于人工种群（ΦST=0.2248）。研究结果认为,伯乐树在物种水平和种群水平都具有较高的遗传多样性,已建立的迁地保护种群不能有效地保护南岭地区天然种群的遗传完整性,建议营建人工迁地保护种群尽可能地从多个不同种群中收集种质材料。 Bretschneidera sinensis, an endemic plant species native to China, is in peril of extinction because of scarce natural genetic resources and human destruction of its natural habitat. In order to assess the genetic integrity of the ex situ conservation populations of this species, the genetic diversity of four natural and two planted populations from Nanling Mountains was investigated using inter-simple sequence repeat （ISSR） markers. A total of 86 discernible DNA fragments were detected with 7 primers, and the 62 fragments were polymorphic. The percentage of polymorphic loci （PPB） and Shannon＇s phenotypic diversity index（HPOP） in a populatio
ISSN:1001-7488