Effect of media components on cell growth and bacterial cellulose production from Acetobacter aceti MTCC 2623

► Comparison of bacterial cellulose production in three different complex media. ► Effect of media composition on structural and spatial arrangement of cellulose. ► FTIR analysis for determination of crystallinity of cellulose. ► Fractional factorial design for bacterial cellulose production from A. aceti MTCC 2623. Acetobacter aceti MTCC 2623 was studied as an alternative microbial source for bacterial cellulose (BC) production. Effect of media components on cell growth rate, BC production and cellulose characteristics were studied. FTIR results showed significant variations in cellulose characteristics produced by A. aceti in different media. Results have shown the role of fermentation time on crystallinity ratio of BC in different media. Further, effect of six different media components on cell growth and BC production was studied using fractional factorial design. Citric acid was found to be the most significant media component for cell growth rate (95% confidence level, R2=0.95). However, direct role of these parameters on cellulose production was not established (p-value>0.05).