G protein-coupled estrogen receptor 1 expression in human vocal fold

Objectives/Hypothesis: Besides the classical sexual receptors, the expression of a recently clarified transmembrane G protein‐coupled receptor (GPR30) has not been reported in the vocal fold so far. We aimed to study whether GPR30 is expressed in the human vocal fold. Study Design: Experimental study using pathologic human vocal fold tissues. Methods: Reverse‐transcription polymerase chain reaction and immunohistochemistry were used to detect the GPR30 expression in a total of 83 vocal fold samples. Results: A total of 66% of the specimens tested were GPR30 mRNA positive, and 54% of the sections were revealed to be GPR30 immunostaining positive in the plasma membrane as well as the nucleus in the epithelial cells. Conclusions: The GPR30 expression both in mRNA and protein level could be detected in the human vocal fold.