A Homogeneous Chemiluminescent Immunoassay Method

A new homogeneous chemiluminescent immunoassay method featuring the use of specific binding members separately labeled with an acridan-based chemiluminescent compound and a peroxidase is reported. Formation of an immunocomplex brings the chemiluminescent compound and the peroxidase into close proxim...

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Veröffentlicht in:	Journal of the American Chemical Society 2013-03, Vol.135 (11), p.4191-4194
Hauptverfasser:	Akhavan-Tafti, Hashem, Binger, Dean G, Blackwood, John J, Chen, Ying, Creager, Richard S, de Silva, Renuka, Eickholt, Robert A, Gaibor, Jose E, Handley, Richard S, Kapsner, Kenneth P, Lopac, Senja K, Mazelis, Michael E, McLernon, Terri L, Mendoza, James D, Odegaard, Bruce H, Reddy, Sarada G, Salvati, Michael, Schoenfelner, Barry A, Shapir, Nir, Shelly, Katherine R, Todtleben, Jeff C, Wang, Guoping, Xie, Wenhua
Format:	Artikel
Sprache:	eng
Schlagworte:	Acridines - chemistry Animals Antibodies, Immobilized - chemistry Cyclic AMP - analysis Horseradish Peroxidase - analysis Humans Immunoassay - methods Interleukin-8 - analysis Luminescent Agents - chemistry Luminescent Measurements - methods Mice Prostate-Specific Antigen - analysis Rats Sheep
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Beschreibung
Zusammenfassung:	A new homogeneous chemiluminescent immunoassay method featuring the use of specific binding members separately labeled with an acridan-based chemiluminescent compound and a peroxidase is reported. Formation of an immunocomplex brings the chemiluminescent compound and the peroxidase into close proximity. Without any separation steps, a chemiluminescent signal is generated upon addition of a trigger solution, and the intensity is directly correlated to the quantity of the analyte.
ISSN:	0002-7863 1520-5126
DOI:	10.1021/ja312039k