Appearance of neural stem cells around the damaged area following traumatic brain injury in aged rats
We have previously reported free radical production after traumatic brain injury (TBI), which induces neural stem cell (NSC) degeneration and death. However, the effects of aging on NSC proliferation around the damaged area following TBI have not been investigated. Therefore, in this study, we used...
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| Veröffentlicht in: | Journal of Neural Transmission 2013-03, Vol.120 (3), p.361-374 |
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| creator | Itoh, Tatsuki Imano, Motohiro Nishida, Shozo Tsubaki, Masahiro Nakayama, Takashi Mizuguchi, Nobuyuki Yamanaka, Shigeaki Tabuchi, Masaki Munakata, Hiroshi Hashimoto, Shigeo Ito, Akihiko Satou, Takao |
| description | We have previously reported free radical production after traumatic brain injury (TBI), which induces neural stem cell (NSC) degeneration and death. However, the effects of aging on NSC proliferation around the damaged area following TBI have not been investigated. Therefore, in this study, we used 10-week (young group) and 24-month-old (aged group) rat TBI models to investigate the effects of aging on NSC proliferation around damaged tissue using immunohistochemical and ex vivo techniques. Young and aged rats received TBI. At 1, 3 and 7 days after TBI, immunohistochemical and lipid peroxidation studies were performed. Immunohistochemistry revealed that the number of nestin-positive cells around the damaged area after TBI in the aged group decreased significantly when compared with those in the young group (
P
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| doi_str_mv | 10.1007/s00702-012-0895-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1315623591</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2894835671</sourcerecordid><originalsourceid>FETCH-LOGICAL-c504t-dbee840095db42b242666875a812736fb0ad4ce35bb7e8d5311badd22da409703</originalsourceid><addsrcrecordid>eNqFkU1rFTEUhoMo9rb1B7iRgBs3Y0--M8tS_CgU3LTrkEzOXOcyk7kmM0j_vRlvFRFESHIgec4bDg8hrxm8ZwDmqtQDeAOsbtuqxjwjOyaFapjU4jnZgQBoWqXlGTkv5QAAjBn7kpxx3irVKrsjeH08os8-dUjnniZcsx9pWXCiHY5joT7Pa4p0-Yo0-snvMdYr9LSfx3H-PqQ9XbJfJ78MHQ3ZD4kO6bDmx1roTzr7pVySF70fC756qhfk4eOH-5vPzd2XT7c313dNp0AuTQyIVgK0KgbJA5dca22N8pZxI3QfwEfZoVAhGLRRCcaCj5Hz6CW0BsQFeXfKPeb524plcdNQtjl8wnktjgmmNBeqZf9HuW1lXaAr-vYv9DCvOdVBNspqZsCYSrET1eW5lIy9O-Zh8vnRMXCbLnfS5aout-lyW8-bp-Q1TBh_d_zyUwF-Akp9SnvMf3z9z9QfNPqe5Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1288617077</pqid></control><display><type>article</type><title>Appearance of neural stem cells around the damaged area following traumatic brain injury in aged rats</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Itoh, Tatsuki ; Imano, Motohiro ; Nishida, Shozo ; Tsubaki, Masahiro ; Nakayama, Takashi ; Mizuguchi, Nobuyuki ; Yamanaka, Shigeaki ; Tabuchi, Masaki ; Munakata, Hiroshi ; Hashimoto, Shigeo ; Ito, Akihiko ; Satou, Takao</creator><creatorcontrib>Itoh, Tatsuki ; Imano, Motohiro ; Nishida, Shozo ; Tsubaki, Masahiro ; Nakayama, Takashi ; Mizuguchi, Nobuyuki ; Yamanaka, Shigeaki ; Tabuchi, Masaki ; Munakata, Hiroshi ; Hashimoto, Shigeo ; Ito, Akihiko ; Satou, Takao</creatorcontrib><description>We have previously reported free radical production after traumatic brain injury (TBI), which induces neural stem cell (NSC) degeneration and death. However, the effects of aging on NSC proliferation around the damaged area following TBI have not been investigated. Therefore, in this study, we used 10-week (young group) and 24-month-old (aged group) rat TBI models to investigate the effects of aging on NSC proliferation around damaged tissue using immunohistochemical and ex vivo techniques. Young and aged rats received TBI. At 1, 3 and 7 days after TBI, immunohistochemical and lipid peroxidation studies were performed. Immunohistochemistry revealed that the number of nestin-positive cells around the damaged area after TBI in the aged group decreased significantly when compared with those in the young group (
P
< 0.01). However, the number of 8-hydroxy-2′-deoxyguanosine-, 4-hydroxy-2-nonenal- and single-stranded DNA (ssDNA)-positive cells and the level of peroxidation around the damaged area after TBI significantly increased in the aged group, compared with those in the young group (
P
< 0.01). Furthermore, almost all ssDNA-positive cells in young and aged groups co-localized with NeuN and nestin staining. Ex vivo studies revealed that neurospheres, which differentiated into neurons and glia in culture, could only be isolated from injured brain tissue in young and aged groups at 3 days after TBI. These results indicate that, although there were fewer NSCs that have the potential to differentiate into neurons and glia, these NSCs escaped free radical-induced degeneration around the damaged area after TBI in the aged rat brain.</description><identifier>ISSN: 0300-9564</identifier><identifier>EISSN: 1435-1463</identifier><identifier>DOI: 10.1007/s00702-012-0895-7</identifier><identifier>PMID: 22955958</identifier><identifier>CODEN: JNTRF3</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Aging ; Aldehydes - analysis ; Animals ; Brain Injuries - metabolism ; Brain Injuries - pathology ; Cell culture ; Cell Differentiation - physiology ; Deoxyguanosine - analogs & derivatives ; Deoxyguanosine - analysis ; Deoxyguanosine - biosynthesis ; Disease Models, Animal ; DNA ; DNA, Single-Stranded - analysis ; Fluorescent Antibody Technique ; Free radicals ; Glia ; Immunohistochemistry ; Intermediate Filament Proteins - analysis ; Intermediate Filament Proteins - biosynthesis ; Lipid peroxidation ; Lipid Peroxidation - physiology ; Male ; Medicine ; Medicine & Public Health ; Nerve Tissue Proteins - analysis ; Nerve Tissue Proteins - biosynthesis ; Nestin ; Neural stem cells ; Neural Stem Cells - cytology ; Neural Stem Cells - metabolism ; Neurodegeneration ; Neurology ; Neurons ; Neurosciences ; neurospheres ; Peroxidation ; Psychiatry ; Rats ; Rats, Wistar ; Translational Neurosciences - Original Article ; Traumatic brain injury</subject><ispartof>Journal of Neural Transmission, 2013-03, Vol.120 (3), p.361-374</ispartof><rights>Springer-Verlag 2012</rights><rights>Springer-Verlag Wien 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-dbee840095db42b242666875a812736fb0ad4ce35bb7e8d5311badd22da409703</citedby><cites>FETCH-LOGICAL-c504t-dbee840095db42b242666875a812736fb0ad4ce35bb7e8d5311badd22da409703</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00702-012-0895-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00702-012-0895-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22955958$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Itoh, Tatsuki</creatorcontrib><creatorcontrib>Imano, Motohiro</creatorcontrib><creatorcontrib>Nishida, Shozo</creatorcontrib><creatorcontrib>Tsubaki, Masahiro</creatorcontrib><creatorcontrib>Nakayama, Takashi</creatorcontrib><creatorcontrib>Mizuguchi, Nobuyuki</creatorcontrib><creatorcontrib>Yamanaka, Shigeaki</creatorcontrib><creatorcontrib>Tabuchi, Masaki</creatorcontrib><creatorcontrib>Munakata, Hiroshi</creatorcontrib><creatorcontrib>Hashimoto, Shigeo</creatorcontrib><creatorcontrib>Ito, Akihiko</creatorcontrib><creatorcontrib>Satou, Takao</creatorcontrib><title>Appearance of neural stem cells around the damaged area following traumatic brain injury in aged rats</title><title>Journal of Neural Transmission</title><addtitle>J Neural Transm</addtitle><addtitle>J Neural Transm (Vienna)</addtitle><description>We have previously reported free radical production after traumatic brain injury (TBI), which induces neural stem cell (NSC) degeneration and death. However, the effects of aging on NSC proliferation around the damaged area following TBI have not been investigated. Therefore, in this study, we used 10-week (young group) and 24-month-old (aged group) rat TBI models to investigate the effects of aging on NSC proliferation around damaged tissue using immunohistochemical and ex vivo techniques. Young and aged rats received TBI. At 1, 3 and 7 days after TBI, immunohistochemical and lipid peroxidation studies were performed. Immunohistochemistry revealed that the number of nestin-positive cells around the damaged area after TBI in the aged group decreased significantly when compared with those in the young group (
P
< 0.01). However, the number of 8-hydroxy-2′-deoxyguanosine-, 4-hydroxy-2-nonenal- and single-stranded DNA (ssDNA)-positive cells and the level of peroxidation around the damaged area after TBI significantly increased in the aged group, compared with those in the young group (
P
< 0.01). Furthermore, almost all ssDNA-positive cells in young and aged groups co-localized with NeuN and nestin staining. Ex vivo studies revealed that neurospheres, which differentiated into neurons and glia in culture, could only be isolated from injured brain tissue in young and aged groups at 3 days after TBI. These results indicate that, although there were fewer NSCs that have the potential to differentiate into neurons and glia, these NSCs escaped free radical-induced degeneration around the damaged area after TBI in the aged rat brain.</description><subject>Aging</subject><subject>Aldehydes - analysis</subject><subject>Animals</subject><subject>Brain Injuries - metabolism</subject><subject>Brain Injuries - pathology</subject><subject>Cell culture</subject><subject>Cell Differentiation - physiology</subject><subject>Deoxyguanosine - analogs & derivatives</subject><subject>Deoxyguanosine - analysis</subject><subject>Deoxyguanosine - biosynthesis</subject><subject>Disease Models, Animal</subject><subject>DNA</subject><subject>DNA, Single-Stranded - analysis</subject><subject>Fluorescent Antibody Technique</subject><subject>Free radicals</subject><subject>Glia</subject><subject>Immunohistochemistry</subject><subject>Intermediate Filament Proteins - analysis</subject><subject>Intermediate Filament Proteins - biosynthesis</subject><subject>Lipid peroxidation</subject><subject>Lipid Peroxidation - physiology</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Nerve Tissue Proteins - analysis</subject><subject>Nerve Tissue Proteins - biosynthesis</subject><subject>Nestin</subject><subject>Neural stem cells</subject><subject>Neural Stem Cells - cytology</subject><subject>Neural Stem Cells - metabolism</subject><subject>Neurodegeneration</subject><subject>Neurology</subject><subject>Neurons</subject><subject>Neurosciences</subject><subject>neurospheres</subject><subject>Peroxidation</subject><subject>Psychiatry</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Translational Neurosciences - Original Article</subject><subject>Traumatic brain injury</subject><issn>0300-9564</issn><issn>1435-1463</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1rFTEUhoMo9rb1B7iRgBs3Y0--M8tS_CgU3LTrkEzOXOcyk7kmM0j_vRlvFRFESHIgec4bDg8hrxm8ZwDmqtQDeAOsbtuqxjwjOyaFapjU4jnZgQBoWqXlGTkv5QAAjBn7kpxx3irVKrsjeH08os8-dUjnniZcsx9pWXCiHY5joT7Pa4p0-Yo0-snvMdYr9LSfx3H-PqQ9XbJfJ78MHQ3ZD4kO6bDmx1roTzr7pVySF70fC756qhfk4eOH-5vPzd2XT7c313dNp0AuTQyIVgK0KgbJA5dca22N8pZxI3QfwEfZoVAhGLRRCcaCj5Hz6CW0BsQFeXfKPeb524plcdNQtjl8wnktjgmmNBeqZf9HuW1lXaAr-vYv9DCvOdVBNspqZsCYSrET1eW5lIy9O-Zh8vnRMXCbLnfS5aout-lyW8-bp-Q1TBh_d_zyUwF-Akp9SnvMf3z9z9QfNPqe5Q</recordid><startdate>20130301</startdate><enddate>20130301</enddate><creator>Itoh, Tatsuki</creator><creator>Imano, Motohiro</creator><creator>Nishida, Shozo</creator><creator>Tsubaki, Masahiro</creator><creator>Nakayama, Takashi</creator><creator>Mizuguchi, Nobuyuki</creator><creator>Yamanaka, Shigeaki</creator><creator>Tabuchi, Masaki</creator><creator>Munakata, Hiroshi</creator><creator>Hashimoto, Shigeo</creator><creator>Ito, Akihiko</creator><creator>Satou, Takao</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope><scope>7TK</scope></search><sort><creationdate>20130301</creationdate><title>Appearance of neural stem cells around the damaged area following traumatic brain injury in aged rats</title><author>Itoh, Tatsuki ; Imano, Motohiro ; Nishida, Shozo ; Tsubaki, Masahiro ; Nakayama, Takashi ; Mizuguchi, Nobuyuki ; Yamanaka, Shigeaki ; Tabuchi, Masaki ; Munakata, Hiroshi ; Hashimoto, Shigeo ; Ito, Akihiko ; Satou, Takao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-dbee840095db42b242666875a812736fb0ad4ce35bb7e8d5311badd22da409703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Aging</topic><topic>Aldehydes - analysis</topic><topic>Animals</topic><topic>Brain Injuries - metabolism</topic><topic>Brain Injuries - pathology</topic><topic>Cell culture</topic><topic>Cell Differentiation - physiology</topic><topic>Deoxyguanosine - analogs & derivatives</topic><topic>Deoxyguanosine - analysis</topic><topic>Deoxyguanosine - biosynthesis</topic><topic>Disease Models, Animal</topic><topic>DNA</topic><topic>DNA, Single-Stranded - analysis</topic><topic>Fluorescent Antibody Technique</topic><topic>Free radicals</topic><topic>Glia</topic><topic>Immunohistochemistry</topic><topic>Intermediate Filament Proteins - analysis</topic><topic>Intermediate Filament Proteins - biosynthesis</topic><topic>Lipid peroxidation</topic><topic>Lipid Peroxidation - physiology</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Nerve Tissue Proteins - analysis</topic><topic>Nerve Tissue Proteins - biosynthesis</topic><topic>Nestin</topic><topic>Neural stem cells</topic><topic>Neural Stem Cells - cytology</topic><topic>Neural Stem Cells - metabolism</topic><topic>Neurodegeneration</topic><topic>Neurology</topic><topic>Neurons</topic><topic>Neurosciences</topic><topic>neurospheres</topic><topic>Peroxidation</topic><topic>Psychiatry</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Translational Neurosciences - Original Article</topic><topic>Traumatic brain injury</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Itoh, Tatsuki</creatorcontrib><creatorcontrib>Imano, Motohiro</creatorcontrib><creatorcontrib>Nishida, Shozo</creatorcontrib><creatorcontrib>Tsubaki, Masahiro</creatorcontrib><creatorcontrib>Nakayama, Takashi</creatorcontrib><creatorcontrib>Mizuguchi, Nobuyuki</creatorcontrib><creatorcontrib>Yamanaka, Shigeaki</creatorcontrib><creatorcontrib>Tabuchi, Masaki</creatorcontrib><creatorcontrib>Munakata, Hiroshi</creatorcontrib><creatorcontrib>Hashimoto, Shigeo</creatorcontrib><creatorcontrib>Ito, Akihiko</creatorcontrib><creatorcontrib>Satou, Takao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>Neurosciences Abstracts</collection><jtitle>Journal of Neural Transmission</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Itoh, Tatsuki</au><au>Imano, Motohiro</au><au>Nishida, Shozo</au><au>Tsubaki, Masahiro</au><au>Nakayama, Takashi</au><au>Mizuguchi, Nobuyuki</au><au>Yamanaka, Shigeaki</au><au>Tabuchi, Masaki</au><au>Munakata, Hiroshi</au><au>Hashimoto, Shigeo</au><au>Ito, Akihiko</au><au>Satou, Takao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Appearance of neural stem cells around the damaged area following traumatic brain injury in aged rats</atitle><jtitle>Journal of Neural Transmission</jtitle><stitle>J Neural Transm</stitle><addtitle>J Neural Transm (Vienna)</addtitle><date>2013-03-01</date><risdate>2013</risdate><volume>120</volume><issue>3</issue><spage>361</spage><epage>374</epage><pages>361-374</pages><issn>0300-9564</issn><eissn>1435-1463</eissn><coden>JNTRF3</coden><abstract>We have previously reported free radical production after traumatic brain injury (TBI), which induces neural stem cell (NSC) degeneration and death. However, the effects of aging on NSC proliferation around the damaged area following TBI have not been investigated. Therefore, in this study, we used 10-week (young group) and 24-month-old (aged group) rat TBI models to investigate the effects of aging on NSC proliferation around damaged tissue using immunohistochemical and ex vivo techniques. Young and aged rats received TBI. At 1, 3 and 7 days after TBI, immunohistochemical and lipid peroxidation studies were performed. Immunohistochemistry revealed that the number of nestin-positive cells around the damaged area after TBI in the aged group decreased significantly when compared with those in the young group (
P
< 0.01). However, the number of 8-hydroxy-2′-deoxyguanosine-, 4-hydroxy-2-nonenal- and single-stranded DNA (ssDNA)-positive cells and the level of peroxidation around the damaged area after TBI significantly increased in the aged group, compared with those in the young group (
P
< 0.01). Furthermore, almost all ssDNA-positive cells in young and aged groups co-localized with NeuN and nestin staining. Ex vivo studies revealed that neurospheres, which differentiated into neurons and glia in culture, could only be isolated from injured brain tissue in young and aged groups at 3 days after TBI. These results indicate that, although there were fewer NSCs that have the potential to differentiate into neurons and glia, these NSCs escaped free radical-induced degeneration around the damaged area after TBI in the aged rat brain.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>22955958</pmid><doi>10.1007/s00702-012-0895-7</doi><tpages>14</tpages></addata></record> |
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| subjects | Aging Aldehydes - analysis Animals Brain Injuries - metabolism Brain Injuries - pathology Cell culture Cell Differentiation - physiology Deoxyguanosine - analogs & derivatives Deoxyguanosine - analysis Deoxyguanosine - biosynthesis Disease Models, Animal DNA DNA, Single-Stranded - analysis Fluorescent Antibody Technique Free radicals Glia Immunohistochemistry Intermediate Filament Proteins - analysis Intermediate Filament Proteins - biosynthesis Lipid peroxidation Lipid Peroxidation - physiology Male Medicine Medicine & Public Health Nerve Tissue Proteins - analysis Nerve Tissue Proteins - biosynthesis Nestin Neural stem cells Neural Stem Cells - cytology Neural Stem Cells - metabolism Neurodegeneration Neurology Neurons Neurosciences neurospheres Peroxidation Psychiatry Rats Rats, Wistar Translational Neurosciences - Original Article Traumatic brain injury |
| title | Appearance of neural stem cells around the damaged area following traumatic brain injury in aged rats |
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