Immune responses and protective efficacy of a recombinant swinepox virus co-expressing HA1 genes of H3N2 and H1N1 swine influenza virus in mice and pigs

The recombinant swine poxvirus rSPV/H3-2A-H1 co-expressing HA1 genes of H3N2 and H1N1 subtype SIV has been constructed and identified. Inoculations of rSPV/H3-2A-H1 yielded ELISA and neutralization antibodies against SIV H1N1 and H3N2, and elicited potent H1N1 and H3N2 SIV-specific INF-γ response fr...

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Veröffentlicht in:	Veterinary microbiology 2013-02, Vol.162 (1), p.259-264
Hauptverfasser:	Xu, Jiarong, Huang, Dongyan, Xu, Jiaping, Liu, Shichao, Lin, Huixing, Zhu, Haodan, Liu, Bao, Chen, Wen, Lu, Chengping
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Neutralizing - immunology Antibodies, Viral - immunology Enzyme-Linked Immunosorbent Assay Female Hemagglutinin Glycoproteins, Influenza Virus - genetics Hemagglutinin Glycoproteins, Influenza Virus - immunology Influenza A Virus, H1N1 Subtype - genetics Influenza A Virus, H1N1 Subtype - immunology Influenza A Virus, H3N2 Subtype - genetics Influenza A Virus, H3N2 Subtype - immunology Influenza Vaccines - genetics Influenza Vaccines - immunology Influenza Vaccines - pharmacology Interferon-gamma - immunology Mice Mice, Inbred BALB C Orthomyxoviridae Infections - immunology Orthomyxoviridae Infections - prevention & control Orthomyxoviridae Infections - veterinary Orthomyxoviridae Infections - virology Poxvirus Protective efficacy Random Allocation Recombinant swinepox virus Suipoxvirus - genetics Suipoxvirus - immunology Sus scrofa Swine Swine Diseases - immunology Swine Diseases - prevention & control Swine Diseases - virology Swine influenza virus Swinepox virus T-Lymphocytes - immunology Vaccines, Synthetic - genetics Vaccines, Synthetic - immunology Vaccines, Synthetic - pharmacology
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Zusammenfassung:	The recombinant swine poxvirus rSPV/H3-2A-H1 co-expressing HA1 genes of H3N2 and H1N1 subtype SIV has been constructed and identified. Inoculations of rSPV/H3-2A-H1 yielded ELISA and neutralization antibodies against SIV H1N1 and H3N2, and elicited potent H1N1 and H3N2 SIV-specific INF-γ response from T-lymphocytes in mice and pigs in this study. Complete protection against SIV H1N1 or H3N2 challenge in pigs was observed.
ISSN:	0378-1135 1873-2542
DOI:	10.1016/j.vetmic.2012.11.026