Comparative analysis of methanogen diversity in the rumen of crossbred buffalo and cattle in the Philippines by using the functional gene mcrA

Comparative analyses of methanogen diversity in the rumen of crossbred buffalo and cattle fed the same diet in the Philippines was performed by cloning the methyl coenzyme M reductase A ( mcrA ) gene. The cattle and buffalo libraries consisted of 50 clones each. Comparative analysis of the amino aci...

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Veröffentlicht in:	Molecular biology reports 2012-12, Vol.39 (12), p.10769-10774
Hauptverfasser:	Lwin, Khin-Ohnmar, Matsui, Hiroki, Ban-Tokuda, Tomomi, Kondo, Makoto, Lapitan, Rosalina M., Herrera, Jesus Rommel V., Del-Barrio, Arnel N., Fujihara, Tsutomu
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animal Anatomy Animal Biochemistry Animals Biomedical and Life Sciences Breeding Buffalo Buffaloes - microbiology Cattle Cattle - microbiology Cloning coenzyme M Comparative studies Crosses, Genetic Diets Digestive system Female Genes, Bacterial - genetics Genetic diversity Genetic Variation Histology Life Sciences Male Methanobacteriales - genetics Methanobrevibacter Methanogenic bacteria Microorganisms Molecular biology Molecular Sequence Data Morphology Oxidoreductases - genetics Philippines Phylogeny reductase Rumen Rumen - microbiology
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Zusammenfassung:	Comparative analyses of methanogen diversity in the rumen of crossbred buffalo and cattle fed the same diet in the Philippines was performed by cloning the methyl coenzyme M reductase A ( mcrA ) gene. The cattle and buffalo libraries consisted of 50 clones each. Comparative analysis of the amino acid sequence revealed that these 2 libraries differed significantly ( P
ISSN:	0301-4851 1573-4978
DOI:	10.1007/s11033-012-1969-1