N-Acetylglucosaminyltransferase V triggers overexpression of MT1-MMP and reinforces the invasive/metastatic potential of cancer cells
► Overexpression of GnT-V leads to upregulation of MT1-MMP in cancer cells. ► The GnT-V-induced upregulation of MT1-MMP promotes cancer invasion. ► MT1-MMP is upregulated in cancer tissues and the extent of upregulation increases as cancer progresses. ► MT1-MMP upregulation shows a relatively strong...
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Veröffentlicht in: | Biochemical and biophysical research communications 2013-02, Vol.431 (4), p.658-663 |
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Sprache: | eng |
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Zusammenfassung: | ► Overexpression of GnT-V leads to upregulation of MT1-MMP in cancer cells. ► The GnT-V-induced upregulation of MT1-MMP promotes cancer invasion. ► MT1-MMP is upregulated in cancer tissues and the extent of upregulation increases as cancer progresses. ► MT1-MMP upregulation shows a relatively strong correlation with GnT-V overexpression in cancer tissues.
N-Acetylglucosaminyltransferase V (GnT-V) is an enzyme that catalyzes the formation of a β1,6-N-acetylglucosamine (GlcNAc) side chain to a core mannosyl residue in N-linked glycoproteins. Besides its direct function of producing aberrant glycoproteins, it promotes cancer progression by its involvement in the stimulation of oncoproteins. Herein, we report that GnT-V guided the transcriptional activation of membrane-type matrix metalloproteinase-1 (MT1-MMP) in cancer cells. The activated MT1-MMP expression had dual effects on cancer progression. It not only promoted proteolytic activity for cancer cells per se, but also led to the activation of MMP-2. Consequently, the activation of the two MMPs triggered by GnT-V intensified the invasive potential. A quantitative analysis using clinical tissues revealed a relatively strong correlation between GnT-V overexpression and MT1-MMP upregulation. In this study, we report for the first time that GnT-V directs cancer progression by modulating MMPs in cancer. |
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ISSN: | 0006-291X 1090-2104 |
DOI: | 10.1016/j.bbrc.2013.01.065 |