Reduction in the earthworm metabolomic response after phenanthrene exposure in soils with high soil organic carbon content

We evaluated the correlation between soil organic carbon (OC) content and metabolic responses of Eisenia fetida earthworms after exposure to phenanthrene (58 ± 3 mg/kg) spiked into seven artificial soils with OC contents ranging from 1 to 27% OC. Principal component analysis of 1H nuclear magnetic resonance (NMR) spectra of aqueous extracts identified statistically significant differences in the metabolic profiles of control and phenanthrene-exposed E. fetida in the 1% OC soil only. Partial least squares analysis identified a metabolic response in the four soils with OC values ≤11% which was well correlated to estimated phenanthrene porewater concentrations. The results suggest that the higher sorption capability of high OC soils decreased the bioavailability of phenanthrene and the subsequent metabolic response of E. fetida. ► Earthworm metabolomic responses correlated to concentrations in soil pore water. ► High organic carbon soils did not elicit any observable metabolomic response. ► Increased sorption to organic matter reduced metabolomic responses in earthworms. 1H NMR-based metabolomics is able to evaluate phenanthrene bioavailability to earthworms in soil.