In silico analysis of the regulatory region of the Yellowtail Kingfish and Zebrafish Kiss and Kiss receptor genes

In silico analysis of the regulatory region of the Yellowtail Kingfish and Zebrafish Kiss and Kiss receptor genes

We have cloned and analysed the partial putative promoter sequences of the Yellowtail Kingfish ( Seriola lalandi ) Kiss2 and Kiss2r genes (380 and 420 bp, respectively). We obtained in silico 1.5 kb of the zebrafish ( Danio rerio ) Kiss1, Kiss2, Kiss1r and zf Kiss2r sequences upstream of the putativ...

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Veröffentlicht in:Fish physiology and biochemistry 2013-02, Vol.39 (1), p.59-63
Hauptverfasser: Nocillado, J. N., Mechaly, A. S., Elizur, A.
Format: Artikel
Sprache:eng
Schlagworte:
Animal Anatomy
Animal Biochemistry
Animal Physiology
Animals
Base Sequence
Binding sites
Biomedical and Life Sciences
Chromosomes
Cloning, Molecular
Computational Biology
Danio rerio
DNA Primers - genetics
Freshwater
Freshwater & Marine Ecology
Genes
Genomes
Histology
Kisspeptins - genetics
Life Sciences
Marine
Molecular Sequence Data
Morphology
Perciformes - genetics
Polymerase Chain Reaction
Receptors, G-Protein-Coupled - genetics
Regulatory Elements, Transcriptional - genetics
Sequence Analysis, DNA
Seriola lalandi
Transcription factors
Vertebrates
Zebrafish - genetics
Zoology
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Beschreibung
Zusammenfassung:We have cloned and analysed the partial putative promoter sequences of the Yellowtail Kingfish ( Seriola lalandi ) Kiss2 and Kiss2r genes (380 and 420 bp, respectively). We obtained in silico 1.5 kb of the zebrafish ( Danio rerio ) Kiss1, Kiss2, Kiss1r and zf Kiss2r sequences upstream of the putative transcriptional initiation site. Bioinformatic analysis revealed promoter regulatory elements including AP-1, Sp1, GR, ER, PR, AR, GATA-1, TTF-1, YY1 and C/EBP. These regulatory elements may mediate novel roles of the Kiss genes and their receptors in addition to their established role in reproductive function.
ISSN:0920-1742
1573-5168
DOI:10.1007/s10695-012-9642-0