Development of a Bioluminescent Bacteria Sheet for the Measurement of Oxygen Concentration

The aim of this study was the preparation and characterization of a bioluminescence bacteria sheet in view of the fabrication of an oxygen marker inside a biofilm. Photobacterium kishitanii sheets were prepared using sodium alginate, and a biofilm of Pseudomonas aeruginosa was dropped over it. The r...

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Veröffentlicht in:	Analytical Sciences 2013/02/10, Vol.29(2), pp.267-269
Hauptverfasser:	SASAKI, Satoshi, MOCHIZUKI, Mika, IGARASHI, Makoto
Format:	Artikel
Sprache:	eng
Schlagworte:	Alginates - chemistry Analytical Chemistry Biofilms Biofilms - growth & development Bioluminescence Biosensing Techniques - methods Chemistry Dissolved oxygen Fabrication Glucuronic Acid - chemistry Hexuronic Acids - chemistry Oxygen Oxygen - metabolism Photobacterium - cytology Photobacterium - metabolism Pseudomonas aeruginosa - physiology Thin films
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Beschreibung
Zusammenfassung:	The aim of this study was the preparation and characterization of a bioluminescence bacteria sheet in view of the fabrication of an oxygen marker inside a biofilm. Photobacterium kishitanii sheets were prepared using sodium alginate, and a biofilm of Pseudomonas aeruginosa was dropped over it. The relationship between the dissolved oxygen (DO) concentration in the biofilm and luminescence from the sheet was examined. As a conclusion, the alginate-gel film method was effective in fabricating a DO-monitoring thin film.
ISSN:	0910-6340 1348-2246
DOI:	10.2116/analsci.29.267