A Simplified Micropatterning Method for Straight-line Neurite Extension of Cultured Hippocampal Neurons

A Simplified Micropatterning Method for Straight-line Neurite Extension of Cultured Hippocampal Neurons

We report a simplified micropatterning method for the straight-line extension of the neurites of cultured neurons. We prepared a poly-D-lysine (PDL)-patterned surface using a polydimethylsiloxane microfluidic stamp. Hippocampal neurons were cultured on the PDL-bound substrate with the stamp removed,...

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Veröffentlicht in:Analytical Sciences 2013/02/10, Vol.29(2), pp.263-266
Hauptverfasser: SUZUKI, Ikuro, NAKAMURA, Kosuke, ODAWARA, Aoi, ALHEBSHI, Amani, GOTOH, Masao
Format: Artikel
Sprache:eng
Schlagworte:
Analytical Chemistry
Animals
Cells, Cultured
Chemistry
Dimethylpolysiloxanes - chemistry
Hippocampus - cytology
Microfluidic Analytical Techniques - methods
Neurites - metabolism
Polylysine - chemistry
Printing - instrumentation
Printing - methods
Rats
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Zusammenfassung:We report a simplified micropatterning method for the straight-line extension of the neurites of cultured neurons. We prepared a poly-D-lysine (PDL)-patterned surface using a polydimethylsiloxane microfluidic stamp. Hippocampal neurons were cultured on the PDL-bound substrate with the stamp removed, allowing for conventional cell seeding and detailed optical observation without fluorescent label. Cultured neurons elongated neurites along straight lines at the single-cell level and displayed spontaneous firing as detected by time-lapse imaging and Ca2+ imaging.
ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.29.263