Biochemical properties of Hemigraphis alternata incorporated chitosan hydrogel scaffold

► Cost effective synthesis of herbal based composite scaffold. ► The scaffold showed an adequate porosity, water uptake and degradation. ► The scaffold posses an effective blood clotting and antibacterial activity. ► The herbal scaffold showed enhanced cell attachment and proliferation. In this work, Hemigraphis alternata extract incorporated chitosan scaffold was synthesized and characterized for wound healing. The antibacterial activity of Hemigraphis incorporated chitosan scaffold (HIC) against Escherichia coli and Staphylococcus aureus was evaluated which showed a reduction in total colony forming units by 45-folds toward E. coli and 25-fold against S. aureus respectively. Cell viability studies using Human Dermal Fibroblast cells (HDF) showed 90% viability even at 48h when compared to the chitosan control. The herbal scaffold made from chitosan was highly haemostatic and antibacterial. The obtained results were in support that the herbal scaffold can be effectively applied for infectious wounds.