Capillary electrophoretic system of ribonucleic acid molecules

▸ A review of CE separations of RNA over the past 20 years. ▸ Summary of electrophoretic conditions and applications of RNA separations. ▸ Contrast RNA CE separations to DNA CE separations, and the rationale behind the differences. ▸ Some future perspectives of the importance of RNA CE separations. Over the past two decades, capillary electrophoresis (CE) has been the subject of extensive development and progress in various DNA based sieving electrophoresis applications, namely Sanger sequencing, forensic short tandem repeat (STR) analysis, clinical genotype screening (SNP), and phylogenetic fingerprinting. Yet, this trend has not been emulated in the RNA field. This review will highlight the development and key analysis parameters of RNA electrophoresis by CE and provide possible explanations for the low research interest in this area.