A naked RNA heptamer targeting the human Bcl-2 mRNA induces apoptosis of HL60 leukemia cells

Highlights ► TRUE gene silencing is a gene control technology based on sgRNA-directed specific mRNA cleavage by tRNase ZL . ► Behavior of heptamer-type sgRNA was investigated to find therapeutic sgRNAs to cure hematological malignancies. ► A heptamer, mh1(Bcl-2), which targets the human Bcl-2 mRNA,...

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Veröffentlicht in:	Cancer letters 2013-01, Vol.328 (2), p.362-368
Hauptverfasser:	Takahashi, Masayuki, Elbarbary, Reyad A, Nakashima, Aiko, Abe, Mayumi, Watanabe, Norihiro, Narita, Miwako, Takahashi, Masuhiro, Tamura, Masato, Yoshida, Tetsuo, Nashimoto, Masayuki
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Apoptosis Apoptosis - genetics Bcl-2 Bcl-2 protein Cancer Cell Line, Tumor Cell Proliferation Female Gene expression Gene Silencing Genes, bcl-2 Hematology Hematology, Oncology and Palliative Medicine HL-60 Cells Humans Leukemia Leukemia - genetics Leukemia - metabolism Malignancy Mice Mice, Nude mRNA Proteins RNA RNA polymerase RNA, Guide - metabolism RNA, Messenger - metabolism Small guide RNA Transfection Transplantation, Heterologous tRNase ZL TRUE gene silencing Vascular endothelial growth factor Xenografts
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Beschreibung
Zusammenfassung:	Highlights ► TRUE gene silencing is a gene control technology based on sgRNA-directed specific mRNA cleavage by tRNase ZL . ► Behavior of heptamer-type sgRNA was investigated to find therapeutic sgRNAs to cure hematological malignancies. ► A heptamer, mh1(Bcl-2), which targets the human Bcl-2 mRNA, can be taken up by cells without any transfection reagents. ► mh1(Bcl-2) can induce apoptosis of HL60 leukemia cells. ► In mouse xenograft experiments, a median survival of the mh1(Bcl-2)-treated mice is longer than that of the control mice.
ISSN:	0304-3835 1872-7980
DOI:	10.1016/j.canlet.2012.10.016