Phenology of Spalangia endius Walker (Hymenoptera, Pteromalidae) in pupae of Musca domestica Linnaeus (Diptera, Muscidae) under laboratory conditions

This work describes the phenology of Spalangia endius Walker in pupae of Musca domestica Linnaeus under laboratory conditions. In order to understand the developmental cycle of Spalangia endius under laboratory conditions, 360 Musca domestica pupae aged from 24 to 48 hours were exposed to 15 S. endius pairs for a period of 24 hours at 26 plus or minus 2 degree C. These pupae were kept in a BOD incubator at the same temperature, with a relative humidity of greater than or equal to 70%, and 12 hours photophase. Fifteen hymenopteran specimens were dissected daily to evaluate their stage and development time. The phenology concluded that S. endius had a development cycle of 19 days with an incubation period of 24 hours. The development of the larvae of S. endius occurred in the subsequent eight days, during which a series of morphological alterations were observed. The pre-pupal stage occurred on the tenth day, where the movement ceased and elimination of the meconium started. The pupal stage occurred from the 11 super(th) to the 19 super(th) day, with emergence of males first, followed by female emergence approximately 24 hours later. These results allowed the evaluation of aspects of the detailed bionomics of the development of S. endius in order to record and program production of this parasitoid, thus optimizing its utilization as a biological control agent.Original Abstract: Objetivando conhecer o ciclo de desenvolvimento de Spalangia endius sob condicoes de laboratorio, 360 pupas de Musca domestica com idade de 24 a 48 horas foram expostas a 15 casais de S. endius por um periodo de 48 horas a 26 plus or minus 2 degree C. Estas pupas foram mantidas em BOD. Com mesma temperatura, umidade relativa greater than or equal to 70% e com fotofase de 12 horas, onde diariamente dissecava-se 15 especimes para avaliar o estagio e tempo de desenvolvimento do himenoptero. A fenologia permite concluir que S. endius apresenta um ciclo de desenvolvimento de 19 dias, cujo periodo de incubacao foi de 24 horas, o desenvolvimento de larvas de S. endius ocorreu nos oito dias subsequentes nos quais uma serie de alteracoes morfologicas foi observada. O estagio de pre-pupa deu-se no decimo dia onde cessa a movimentacao e inicia a eliminacao de meconio. O estagio pupal ocorreu do decimo primeiro dia ao decimo nono, momento da emergencia dos primeiros machos, ja as femeas iniciaram a emergencia aproximadamente 24 horas apos. Estes resultados permitem avaliar aspectos da bionomia po