DETECTION OF VIRULENCE GENES IN ESCHERICHIA COLI STRAINS ISOLATED FROM DIARRHEIC AND HEALTHY FECES OF DAIRY CALVES IN BRAZIL
The aim of this work was to test 101 strains of E. coli for virulence factors associated with enterotoxigenic and enterohemorrhagic pathotypes of E. coli isolated from diarrheic and non-diarrheic calves. The virulence factors of E. coliStx1 (Shiga toxin), Stx2, Ehly (Enterohemolysin), the eae gene,...
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| Veröffentlicht in: | Arquivos do Instituto Biológico (São Paulo) 2012-06, Vol.79 (2), p.273-276 |
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| description | The aim of this work was to test 101 strains of E. coli for virulence factors associated with enterotoxigenic and enterohemorrhagic pathotypes of E. coli isolated from diarrheic and non-diarrheic calves. The virulence factors of E. coliStx1 (Shiga toxin), Stx2, Ehly (Enterohemolysin), the eae gene, LT-II (heat-labile enterotoxin), STa (heat-stable toxin), and adhesins K99 and F41 were detected by PCR. Serogroups were determined by serological methods and Stx production was observed by biological assays in Vero cells. The frequency of the eae gene was higher in isolates from diarrheic calves (35/58,60.3%) than in non-diarrheic calves (8/43,18.6%; P |
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| fullrecord | <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_miscellaneous_1285091373</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1285091373</sourcerecordid><originalsourceid>FETCH-proquest_miscellaneous_12850913733</originalsourceid><addsrcrecordid>eNqVzM1KAzEUhuEsFKy293CW3RTShunP8vTkjDkQE8ikhboppYygjLY1dufFO6A34OpbvB_PjRpoPdMTM6_Mnbov5U3rqtLL-UB9W85MWWKAWMNW0sZzIIZHDtyABOCGHCchJwgUvUCTE0roWxM9ZrZQp_gEVjAlx0KAwYJj9NntoGbqlR62KGkHhH77q64TPosfqtuXQ1fa0d8-qHHNmdzk_Hm6XNvytX9_Lce26w4f7ela9tPZstKrqVkY84_rD01xRdg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1285091373</pqid></control><display><type>article</type><title>DETECTION OF VIRULENCE GENES IN ESCHERICHIA COLI STRAINS ISOLATED FROM DIARRHEIC AND HEALTHY FECES OF DAIRY CALVES IN BRAZIL</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>de Moura, C ; Ludovico, M ; Valadares, G F ; Gatti, MSV ; Leite, D S</creator><creatorcontrib>de Moura, C ; Ludovico, M ; Valadares, G F ; Gatti, MSV ; Leite, D S</creatorcontrib><description>The aim of this work was to test 101 strains of E. coli for virulence factors associated with enterotoxigenic and enterohemorrhagic pathotypes of E. coli isolated from diarrheic and non-diarrheic calves. The virulence factors of E. coliStx1 (Shiga toxin), Stx2, Ehly (Enterohemolysin), the eae gene, LT-II (heat-labile enterotoxin), STa (heat-stable toxin), and adhesins K99 and F41 were detected by PCR. Serogroups were determined by serological methods and Stx production was observed by biological assays in Vero cells. The frequency of the eae gene was higher in isolates from diarrheic calves (35/58,60.3%) than in non-diarrheic calves (8/43,18.6%; P<0.001). The gene forStx1 occurred at high frequencies in the diarrheic strains (24/58,41.3%) as well as in non-diarrheic (19/43, 44.2%) ones and all strains that were Stx positive by PCR showed cytotoxicity in Vero cells. Stx2 was found in ten strains, Ehly in eight strains, and LT-II in only two strains. Twenty-eight strains were negative for all of the PCR assays, including for F41 and K99 adhesins. The serogroups O7, O23, 04, 08, 0153 and O156 were observed most frequently. Our results show that strains of E. coli isolated from cattle have similar virulence factors genes to strains isolated from cases of diseases in humans and may be a source of potentially pathogenic STEC for humans.Original Abstract: O objetivo deste trabalho foi detectar em 101 amostras de E. coli isoladas de bezerros com e sem diarreia, fatores de virulencia associados aos patotipos de E. coli enterotoxigenica e enterohemorragica. Os fatores de virulencia de E. coli Stx1 (Shiga toxina), Stx2, Ehly (Enterohemolisina), o gene eae, LT-II (enterotoxina termolabil), STa (toxina termo-estavel), e adesinas K99 e F41 foram detectados por PCR. Os sorogrupos foram determinados por metodos sorologicos e a producao de Stx foi observada atraves de ensaios biologicos em celulas Vero. A frequencia de deteccao do gene eae foi maior nos isolados de bezerros com diarreia (35/58, 60,3%) do que em bezerros saudaveis (8/43, 18,6%; P < 0.001). O gene da toxina Stx1 foi detectado em alta frequilncia em amostras diarreicas (24/58, 40,3%), bem como em amostras nao diarreicas (19/43, 44,2%) e todas as amostras positivas para toxina Stx em PCR mostraram citotoxicidade em celulas Vero. Stx2 foi encontrada em dez amostras, Ehly em oito amostras, e LT-II em duas amostras. Vinte e seis amostras foram negativas para todos os ensaios de PCR, incluindo para as adesinas F41 e K99. Os sorogrupos O7,023,04,08,0153eO156 foram detectados com maior frequencia. O trabalho mostra que amostras de E. coli isoladas de bovinos apresentam fatores de viruldncia semelhantes a isolados de casos de doencas em humanos e possivelmente e uma fonte para STEC potencialmente patogenicas para humanos.</description><identifier>ISSN: 0020-3653</identifier><language>eng</language><subject>Adhesins ; Cytotoxicity ; Dairies ; eae gene ; enterohemolysin ; Escherichia coli ; Feces ; heat-labile enterotoxin ; Polymerase chain reaction ; Shiga toxin ; Thermal stability ; Vero cells ; virulence factors</subject><ispartof>Arquivos do Instituto Biológico (São Paulo), 2012-06, Vol.79 (2), p.273-276</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids></links><search><creatorcontrib>de Moura, C</creatorcontrib><creatorcontrib>Ludovico, M</creatorcontrib><creatorcontrib>Valadares, G F</creatorcontrib><creatorcontrib>Gatti, MSV</creatorcontrib><creatorcontrib>Leite, D S</creatorcontrib><title>DETECTION OF VIRULENCE GENES IN ESCHERICHIA COLI STRAINS ISOLATED FROM DIARRHEIC AND HEALTHY FECES OF DAIRY CALVES IN BRAZIL</title><title>Arquivos do Instituto Biológico (São Paulo)</title><description>The aim of this work was to test 101 strains of E. coli for virulence factors associated with enterotoxigenic and enterohemorrhagic pathotypes of E. coli isolated from diarrheic and non-diarrheic calves. The virulence factors of E. coliStx1 (Shiga toxin), Stx2, Ehly (Enterohemolysin), the eae gene, LT-II (heat-labile enterotoxin), STa (heat-stable toxin), and adhesins K99 and F41 were detected by PCR. Serogroups were determined by serological methods and Stx production was observed by biological assays in Vero cells. The frequency of the eae gene was higher in isolates from diarrheic calves (35/58,60.3%) than in non-diarrheic calves (8/43,18.6%; P<0.001). The gene forStx1 occurred at high frequencies in the diarrheic strains (24/58,41.3%) as well as in non-diarrheic (19/43, 44.2%) ones and all strains that were Stx positive by PCR showed cytotoxicity in Vero cells. Stx2 was found in ten strains, Ehly in eight strains, and LT-II in only two strains. Twenty-eight strains were negative for all of the PCR assays, including for F41 and K99 adhesins. The serogroups O7, O23, 04, 08, 0153 and O156 were observed most frequently. Our results show that strains of E. coli isolated from cattle have similar virulence factors genes to strains isolated from cases of diseases in humans and may be a source of potentially pathogenic STEC for humans.Original Abstract: O objetivo deste trabalho foi detectar em 101 amostras de E. coli isoladas de bezerros com e sem diarreia, fatores de virulencia associados aos patotipos de E. coli enterotoxigenica e enterohemorragica. Os fatores de virulencia de E. coli Stx1 (Shiga toxina), Stx2, Ehly (Enterohemolisina), o gene eae, LT-II (enterotoxina termolabil), STa (toxina termo-estavel), e adesinas K99 e F41 foram detectados por PCR. Os sorogrupos foram determinados por metodos sorologicos e a producao de Stx foi observada atraves de ensaios biologicos em celulas Vero. A frequencia de deteccao do gene eae foi maior nos isolados de bezerros com diarreia (35/58, 60,3%) do que em bezerros saudaveis (8/43, 18,6%; P < 0.001). O gene da toxina Stx1 foi detectado em alta frequilncia em amostras diarreicas (24/58, 40,3%), bem como em amostras nao diarreicas (19/43, 44,2%) e todas as amostras positivas para toxina Stx em PCR mostraram citotoxicidade em celulas Vero. Stx2 foi encontrada em dez amostras, Ehly em oito amostras, e LT-II em duas amostras. Vinte e seis amostras foram negativas para todos os ensaios de PCR, incluindo para as adesinas F41 e K99. Os sorogrupos O7,023,04,08,0153eO156 foram detectados com maior frequencia. O trabalho mostra que amostras de E. coli isoladas de bovinos apresentam fatores de viruldncia semelhantes a isolados de casos de doencas em humanos e possivelmente e uma fonte para STEC potencialmente patogenicas para humanos.</description><subject>Adhesins</subject><subject>Cytotoxicity</subject><subject>Dairies</subject><subject>eae gene</subject><subject>enterohemolysin</subject><subject>Escherichia coli</subject><subject>Feces</subject><subject>heat-labile enterotoxin</subject><subject>Polymerase chain reaction</subject><subject>Shiga toxin</subject><subject>Thermal stability</subject><subject>Vero cells</subject><subject>virulence factors</subject><issn>0020-3653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqVzM1KAzEUhuEsFKy293CW3RTShunP8vTkjDkQE8ikhboppYygjLY1dufFO6A34OpbvB_PjRpoPdMTM6_Mnbov5U3rqtLL-UB9W85MWWKAWMNW0sZzIIZHDtyABOCGHCchJwgUvUCTE0roWxM9ZrZQp_gEVjAlx0KAwYJj9NntoGbqlR62KGkHhH77q64TPosfqtuXQ1fa0d8-qHHNmdzk_Hm6XNvytX9_Lce26w4f7ela9tPZstKrqVkY84_rD01xRdg</recordid><startdate>20120601</startdate><enddate>20120601</enddate><creator>de Moura, C</creator><creator>Ludovico, M</creator><creator>Valadares, G F</creator><creator>Gatti, MSV</creator><creator>Leite, D S</creator><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20120601</creationdate><title>DETECTION OF VIRULENCE GENES IN ESCHERICHIA COLI STRAINS ISOLATED FROM DIARRHEIC AND HEALTHY FECES OF DAIRY CALVES IN BRAZIL</title><author>de Moura, C ; Ludovico, M ; Valadares, G F ; Gatti, MSV ; Leite, D S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_miscellaneous_12850913733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adhesins</topic><topic>Cytotoxicity</topic><topic>Dairies</topic><topic>eae gene</topic><topic>enterohemolysin</topic><topic>Escherichia coli</topic><topic>Feces</topic><topic>heat-labile enterotoxin</topic><topic>Polymerase chain reaction</topic><topic>Shiga toxin</topic><topic>Thermal stability</topic><topic>Vero cells</topic><topic>virulence factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Moura, C</creatorcontrib><creatorcontrib>Ludovico, M</creatorcontrib><creatorcontrib>Valadares, G F</creatorcontrib><creatorcontrib>Gatti, MSV</creatorcontrib><creatorcontrib>Leite, D S</creatorcontrib><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Arquivos do Instituto Biológico (São Paulo)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>de Moura, C</au><au>Ludovico, M</au><au>Valadares, G F</au><au>Gatti, MSV</au><au>Leite, D S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DETECTION OF VIRULENCE GENES IN ESCHERICHIA COLI STRAINS ISOLATED FROM DIARRHEIC AND HEALTHY FECES OF DAIRY CALVES IN BRAZIL</atitle><jtitle>Arquivos do Instituto Biológico (São Paulo)</jtitle><date>2012-06-01</date><risdate>2012</risdate><volume>79</volume><issue>2</issue><spage>273</spage><epage>276</epage><pages>273-276</pages><issn>0020-3653</issn><abstract>The aim of this work was to test 101 strains of E. coli for virulence factors associated with enterotoxigenic and enterohemorrhagic pathotypes of E. coli isolated from diarrheic and non-diarrheic calves. The virulence factors of E. coliStx1 (Shiga toxin), Stx2, Ehly (Enterohemolysin), the eae gene, LT-II (heat-labile enterotoxin), STa (heat-stable toxin), and adhesins K99 and F41 were detected by PCR. Serogroups were determined by serological methods and Stx production was observed by biological assays in Vero cells. The frequency of the eae gene was higher in isolates from diarrheic calves (35/58,60.3%) than in non-diarrheic calves (8/43,18.6%; P<0.001). The gene forStx1 occurred at high frequencies in the diarrheic strains (24/58,41.3%) as well as in non-diarrheic (19/43, 44.2%) ones and all strains that were Stx positive by PCR showed cytotoxicity in Vero cells. Stx2 was found in ten strains, Ehly in eight strains, and LT-II in only two strains. Twenty-eight strains were negative for all of the PCR assays, including for F41 and K99 adhesins. The serogroups O7, O23, 04, 08, 0153 and O156 were observed most frequently. Our results show that strains of E. coli isolated from cattle have similar virulence factors genes to strains isolated from cases of diseases in humans and may be a source of potentially pathogenic STEC for humans.Original Abstract: O objetivo deste trabalho foi detectar em 101 amostras de E. coli isoladas de bezerros com e sem diarreia, fatores de virulencia associados aos patotipos de E. coli enterotoxigenica e enterohemorragica. Os fatores de virulencia de E. coli Stx1 (Shiga toxina), Stx2, Ehly (Enterohemolisina), o gene eae, LT-II (enterotoxina termolabil), STa (toxina termo-estavel), e adesinas K99 e F41 foram detectados por PCR. Os sorogrupos foram determinados por metodos sorologicos e a producao de Stx foi observada atraves de ensaios biologicos em celulas Vero. A frequencia de deteccao do gene eae foi maior nos isolados de bezerros com diarreia (35/58, 60,3%) do que em bezerros saudaveis (8/43, 18,6%; P < 0.001). O gene da toxina Stx1 foi detectado em alta frequilncia em amostras diarreicas (24/58, 40,3%), bem como em amostras nao diarreicas (19/43, 44,2%) e todas as amostras positivas para toxina Stx em PCR mostraram citotoxicidade em celulas Vero. Stx2 foi encontrada em dez amostras, Ehly em oito amostras, e LT-II em duas amostras. Vinte e seis amostras foram negativas para todos os ensaios de PCR, incluindo para as adesinas F41 e K99. Os sorogrupos O7,023,04,08,0153eO156 foram detectados com maior frequencia. O trabalho mostra que amostras de E. coli isoladas de bovinos apresentam fatores de viruldncia semelhantes a isolados de casos de doencas em humanos e possivelmente e uma fonte para STEC potencialmente patogenicas para humanos.</abstract></addata></record> |
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| subjects | Adhesins Cytotoxicity Dairies eae gene enterohemolysin Escherichia coli Feces heat-labile enterotoxin Polymerase chain reaction Shiga toxin Thermal stability Vero cells virulence factors |
| title | DETECTION OF VIRULENCE GENES IN ESCHERICHIA COLI STRAINS ISOLATED FROM DIARRHEIC AND HEALTHY FECES OF DAIRY CALVES IN BRAZIL |
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