DETECTION OF VIRULENCE GENES IN ESCHERICHIA COLI STRAINS ISOLATED FROM DIARRHEIC AND HEALTHY FECES OF DAIRY CALVES IN BRAZIL

DETECTION OF VIRULENCE GENES IN ESCHERICHIA COLI STRAINS ISOLATED FROM DIARRHEIC AND HEALTHY FECES OF DAIRY CALVES IN BRAZIL

The aim of this work was to test 101 strains of E. coli for virulence factors associated with enterotoxigenic and enterohemorrhagic pathotypes of E. coli isolated from diarrheic and non-diarrheic calves. The virulence factors of E. coliStx1 (Shiga toxin), Stx2, Ehly (Enterohemolysin), the eae gene,...

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Veröffentlicht in:Arquivos do Instituto Biológico (São Paulo) 2012-06, Vol.79 (2), p.273-276
Hauptverfasser: de Moura, C, Ludovico, M, Valadares, G F, Gatti, MSV, Leite, D S
Format: Artikel
Sprache:eng
Schlagworte:
Adhesins
Cytotoxicity
Dairies
eae gene
enterohemolysin
Escherichia coli
Feces
heat-labile enterotoxin
Polymerase chain reaction
Shiga toxin
Thermal stability
Vero cells
virulence factors
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Zusammenfassung:The aim of this work was to test 101 strains of E. coli for virulence factors associated with enterotoxigenic and enterohemorrhagic pathotypes of E. coli isolated from diarrheic and non-diarrheic calves. The virulence factors of E. coliStx1 (Shiga toxin), Stx2, Ehly (Enterohemolysin), the eae gene, LT-II (heat-labile enterotoxin), STa (heat-stable toxin), and adhesins K99 and F41 were detected by PCR. Serogroups were determined by serological methods and Stx production was observed by biological assays in Vero cells. The frequency of the eae gene was higher in isolates from diarrheic calves (35/58,60.3%) than in non-diarrheic calves (8/43,18.6%; P
ISSN:0020-3653