Effects of amino acids and vitamins on the ultrastructure of the hypothalamus and neurotransmitter in exhausted rats

Objective To investigate the effects of amino acids and vitamins on the ultrastructure of the hypothalamus and neurotransmitter in exhausted rats. Methods After adaptive swimming, 36 male SD rats were randomly divided into three groups, namely, capsule, control, and granules, with 12 rats in each group. Rats in 3 groups were given respectively amino acids capsule (8 kinds of essential amino acids and 11 kinds of vitamins were contained), normal drinking water, or amino acid-fructose beverage (2.5ml/100g, 2 times per day by gavage for 14 days). Exhaustion of rats was produced by non-loading swimming. The duration of the experiment lasted 14 days. After the last exhaustive swimming, the hypothalamus of the rats was removed for the observation of its ultrastructure under electron microscope. The contents of 5-hydroxytryptamine (5-HT), 5-hydroxyindole acetic acid (5HIAA), hydroxyphenyl acetic acid (HOPAC), and γ-aminobutyric acid (GABA) in the hypothalamus were measured with high performance liquid chromatography